ArticleJournal of dental research2024
Injectable Tissue-Specific Hydrogel System for Pulp-Dentin Regeneration.
Article in Journal of dental research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Strategies to Microengineer Vascularized, Mineralized, and Immunomodulatory Niches for Dental Regeneration.Journal of endodontics · 2026Article
- [Frontier research on smart delivery biomaterials in the field of oral tissue engineering].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2026Review
- Decellularized Dental Pulp Matrix Hydrogel Promotes Functional Endodontic Regeneration In Situ.International endodontic journal · 2026Article
- Mechanical Signaling in the Microenvironment Regulates the Differentiation of Dental Pulp Stem Cells: A Novel Strategy For Pulp-Dentin Complex Regeneration.Stem cell reviews and reports · 2026Review
- Smart Hydrogels for Craniofacial Regeneration.Cells · 2026Review
- Collagen Scaffolds in Regenerative Endodontic Procedures: Current Evidence, Limitations, and Future Perspectives.Polymers · 2026Review
- Canonical and Noncanonical WNT-Loaded Hydrogel for Dentin-Pulp Regeneration.Journal of dental research · 2026Article
- Collagen-Based Delivery Systems for the Prevention of Alveolitis: A Narrative Review and Rationale for Biopharmaceutical Development Requirements.Journal of functional biomaterials · 2026Review
- Neuro-Instructive Hydrogels - A Key to Sensory Innervation in Oral Tissues: A Comprehensive Review.International journal of nanomedicine · 2026Review
- Recent Advances in Injectable Hydrogel Biotherapeutics for Regenerative Dental Medicine.Macromolecular bioscience · 2025Review
- Next-Generation Natural Hydrogels in Oral Tissue Engineering.Pharmaceutics · 2025Review
- Article
- Three-dimensional matrix stiffness-based stem cell soil: Tri-phase biomechanical structure promoted human dental pulp stem cells to achieve pulpodentin regeneration.Materials today. Bio · 2025Article
- Guidance for evaluating biomaterials' properties and biological potential for dental pulp tissue engineering and regeneration research.Dental materials : official publication of the Academy of Dental Materials · 2025Review
- Evaluation of Physicochemical Properties of Polymeric Systems for Potential Applications in Cartilage Tissue Engineering.International journal of molecular sciences · 2025Article
- Review
- PDGF-BB overexpressing dental pulp stem cells improve angiogenesis in dental pulp regeneration.Frontiers in bioengineering and biotechnology · 2025Article
- Aluminum-Free Borosilicate Glass Functionalized Hydrogels for Enhanced Dental Tissue Regeneration.Materials (Basel, Switzerland) · 2024Article
- Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study.BMC oral health · 2024Article
- Global trends in clinical trials involving engineered biomaterials.Science advances · 2024Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
The quest for finding a suitable scaffold system that supports cell survival and function and, ultimately, the regeneration of the pulp-dentin complex remains challenging. Herein, we hypothesized that dental pulp stem cells (DPSCs) encapsulated in a collagen-based hydrogel with varying stiffness would regenerate functional dental pulp and dentin when concentrically injected into the tooth slices. Collagen hydrogels with concentrations of 3 mg/mL (Col3) and 10 mg/mL (Col10) were prepared, and their stiffness and microstructure were assessed using a rheometer and scanning electron microscopy, respectively. DPSCs were then encapsulated in the hydrogels, and their viability and differentiation capacity toward endothelial and odontogenic lineages were evaluated using live/dead assay and quantitative real-time polymerase chain reaction. For in vivo experiments, DPSC-encapsulated collagen hydrogels with different stiffness, with or without growth factors, were injected into pulp chambers of dentin tooth slices and implanted subcutaneously in severe combined immunodeficient (SCID) mice. Specifically, vascular endothelial growth factor (VEGF [50 ng/mL]) was loaded into Col3 and bone morphogenetic protein (BMP2 [50 ng/mL]) into Col10. Pulp-dentin regeneration was evaluated by histological and immunofluorescence staining. Data were analyzed using 1-way or 2-way analysis of variance accordingly (α = 0.05). Rheology and microscopy data revealed that Col10 had a stiffness of 8,142 Pa with a more condensed and less porous structure, whereas Col3 had a stiffness of 735 Pa with a loose microstructure. Furthermore, both Col3 and Col10 supported DPSCs' survival. Quantitative polymerase chain reaction showed Col3 promoted significantly higher von Willebrand factor (VWF) and CD31 expression after 7 and 14 d under endothelial differentiation conditions (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.