Evidence map›Paper›PMID 38409720›Full record

ArticleCurrent pharmaceutical biotechnology2024

Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead.

Henrique Bonifácio Moura, Henrique Gama Ker, Luana S Ramos, Lais M Nogueira, Juliana M Machado, Daniel S Dias, Rita Alaíde Leandro Rodrigues, Mariana Campos da Paz, Rafael Gonçalves Teixeira-Neto, Alexandre Barbosa Reis and 2 more

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Article in Current pharmaceutical biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Henrique Bonifácio MouraLaboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501- 296, Brazil.
Henrique Gama KerLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, MG, 35400-000, Brazil.
Luana S RamosLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Lais M NogueiraLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Juliana M MachadoLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Daniel S DiasLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Rita Alaíde Leandro RodriguesLaboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501- 296, Brazil.
Mariana Campos da PazLaboratório de Nanobiotecnologia & Bioativos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Rafael Gonçalves Teixeira-NetoLaboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501- 296, Brazil.
Alexandre Barbosa ReisLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, MG, 35400-000, Brazil.
Alexsandro S GaldinoLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.
Eduardo S SilvaLaboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501- 296, Brazil.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 435224/2018-2Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundação de Amparo à Pesquisa do Estado de Minas Gerais 02556-18,APQ 02577-18,INV-00037-14,APQ-02638-17,APQ-01008-14,APQ-01373-14,APQ-03505-13Universidade Federal de Ouro Preto 082017080
6 · The paper itself

Abstract

backgroundVisceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the

objectiveTo develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis.

methodsThe current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH.

resultsIn the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs' clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with

conclusionThe flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.

Indexed as

Antigens, ProtozoanDog DiseasesFlow CytometryLeishmaniasis, VisceralAnimalsAntibodies, ProtozoanCross ReactionsDogsEpitopesLeishmania infantumSensitivity and SpecificitySerologic TestsAntibodies, ProtozoanAntigens, ProtozoanEpitopescanine visceral leishmaniasisdiagnosisdogsflow cytometry serologyLeishmania infantumzoonotic disease.

Identifiers

PMID38409720

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.