ArticleBiology of reproduction2024
In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.
Article in Biology of reproduction, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- In vitro processes alter the embryonic disc epigenome and transcriptome in the pre-implantation elongated bovine embryo†.Biology of reproduction · 2026Article
- Effects of leonurine supplementation during in vitro culture on oxidative stress, cell proliferation, apoptosis, and autophagy in bovine embryos.Scientific reports · 2026Article
- When bovine life begins in a petri dish: transcriptomic insights from embryos to calves conceived in vitro.The Journal of reproduction and development · 2026Review
- Bovine Endometrium Drives and Responds to Divergence of In Vitro Produced Conceptus Biochemistry.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation.Cell reports · 2025Article
- METTL7A improves bovine IVF embryo competence by attenuating oxidative stress†.Biology of reproduction · 2025Article
- Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates.Scientific reports · 2025Article
- Molecular factors driving the development of bovine embryos and embryo-like structures.Animal reproduction · 2025Review
- METTL7A improves bovine IVF embryo competence by attenuating oxidative stress.bioRxiv : the preprint server for biology · 2024Article
- Evolution of Media Supporting the Development of Mammalian Preimplantation Embryos In Vitro.Biology · 2024Review
- Bovine embryo productionAnimal reproduction · 2024Review
Corrections and comments
- Update of
Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
Abstract
Profiling bovine blastocyst transcriptome at the single-cell level has enabled us to reveal the first cell lineage segregation, during which the inner cell mass (ICM), trophectoderm (TE), and an undefined population of transitional cells were identified. By comparing the transcriptome of blastocysts derived in vivo (IVV), in vitro from a conventional culture medium (IVC), and in vitro from an optimized reduced nutrient culture medium (IVR), we found a delay of the cell fate commitment to ICM in the IVC and IVR embryos. Developmental potential differences between IVV, IVC, and IVR embryos were mainly contributed by ICM and transitional cells. Pathway analysis of these non-TE cells between groups revealed highly active metabolic and biosynthetic processes, reduced cellular signaling, and reduced transmembrane transport activities in IVC embryos that may lead to reduced developmental potential. IVR embryos had lower activities in metabolic and biosynthetic processes but increased cellular signaling and transmembrane transport, suggesting these cellular mechanisms may contribute to improved blastocyst development compared to IVC embryos. However, the IVR embryos had compromised development compared to IVV embryos with notably over-active transmembrane transport activities that impaired ion homeostasis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.