Evidence map›Paper›PMID 38405786›Full record

ArticlebioRxiv : the preprint server for biology2023

Torques within and outside the human spindle balance twist at anaphase.

Lila Neahring, Yifei He, Nathan H Cho, Gaoxiang Liu, Jonathan Fernandes, Caleb J Rux, Konstantinos Nakos, Radhika Subramanian, Srigokul Upadhyayula, Ahmet Yildiz and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Lila NeahringDepartment of Bioengineering & Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0003-2272-8732
Yifei HeDepartment of Chemistry, University of California Berkeley, Berkeley, CA, USA.
Nathan H ChoDepartment of Bioengineering & Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0002-0110-1343
Gaoxiang LiuDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.ORCID 0000-0002-1001-0064
Jonathan FernandesDepartment of Chemistry, University of California Berkeley, Berkeley, CA, USA.ORCID 0000-0002-3422-1763
Caleb J RuxDepartment of Bioengineering & Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0002-7020-8611
Konstantinos NakosDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA.
Radhika SubramanianDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA.ORCID 0000-0002-3011-9403
Srigokul UpadhyayulaDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.ORCID 0000-0001-6727-5883
Ahmet YildizDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.ORCID 0000-0003-4792-174X
Sophie DumontDepartment of Bioengineering & Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0002-8283-1523
University of California, Berkeley · USUniversity of California, San Francisco · USHarvard University · USLawrence Berkeley National Laboratory · US

Funding

Microscopy system upgradeR35GM136420 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Sophie Dumont · 2020 to 2026
$4.6M
Understanding the Mechanism and Regulation of the Human Cytoplasmic Dynein ComplexR01GM094522 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI YILDIZ, AHMET · 2011 to 2019
$2.8M
Defining the mechanisms by which NuMA drives spindle mechanical robustnessF31CA275394 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHO, NATHAN · 2023 to 2025
$89k
NCI NIH HHS F31 CA275394NIGMS NIH HHS R01 GM094522NIGMS NIH HHS R35 GM136420
6 · The paper itself

Abstract

At each cell division, nanometer-scale motors and microtubules give rise to the micron-scale spindle. Many mitotic motors step helically around microtubules in vitro, and most are predicted to twist the spindle in a left-handed direction. However, the human spindle exhibits only slight global twist, raising the question of how these molecular torques are balanced. Here, using lattice light sheet microscopy, we find that anaphase spindles in the epithelial cell line MCF10A have a high baseline twist, and we identify factors that both increase and decrease this twist. The midzone motors KIF4A and MKLP1 are redundantly required for left-handed twist at anaphase, and we show that KIF4A generates left-handed torque in vitro. The actin cytoskeleton also contributes to left-handed twist, but dynein and its cortical recruitment factor LGN counteract it. Together, our work demonstrates that force generators regulate twist in opposite directions from both within and outside the spindle, preventing strong spindle twist during chromosome segregation.

Indexed as

chiralitycortexdyneinlattice light sheet microscopymitosismotorsspindletorquetwist

Identifiers

PMID38405786
PMCPMC10888964
OpenAlexW4389570369

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.