Evidence map›Paper›PMID 38403791›Full record

ArticleMolecular biology reports2024

Unveiling the dynamic expression of PR-1 during Musa spp. infection by Fusarium oxysporum fsp. Cubense: a cloning and characterization study.

Chelliah Anuradha, Punchakkara Prashina Mol, Arumugam Chandrasekar, Suthanthiram Backiyarani, Raman Thangavelu, Ramasamy Selvarajan

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Banana breeding by genome design.Journal of integrative plant biology · 2025
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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Chelliah AnuradhaICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India. anuradha.chelliah@gmail.com.ORCID http://orcid.org/0000-0002-8534-9345
Punchakkara Prashina MolICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India.
Arumugam ChandrasekarICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India.
Suthanthiram BackiyaraniICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India.
Raman ThangaveluICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India.
Ramasamy SelvarajanICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, Tamil Nadu, 620 102, India.
National Research Centre for Banana · IN

Funding

Indian Council of Agricultural Research IXX14668
6 · The paper itself

Abstract

backgroundPathogen-related proteins (PR) are pivotal in plant defense, combating diverse biotic and abiotic stresses. While multiple gene families contribute to banana resistance against Fusarium oxysporum f sp. cubense (Foc), Pseudocercospora eumusae, and Pratylenchus coffeae, the significance of PR-1 genes in defense is paramount.

methodsThree PR-1 genes, up-regulated under diverse biotic stresses, were cloned from both resistant and susceptible cultivars of Foc, P. eumusae, and P. coffeae. Molecular characterization, phylogenetic analysis, and docking studies with the Foc TR4 CP gene were conducted.

resultsThrough transcriptomic and real-time studies, three PR-1 genes (Ma02_g15050, Ma02_g15060, and Ma04_g34800) from Musa spp. were identified. These genes exhibited significant up-regulation in resistant cultivars when exposed to Foc, P. eumusae, and P. coffeae. Cloning of these genes was successfully performed from both resistant and susceptible cultivars of Foc race 1 and TR4, P. eumusae, and P. coffeae. Distinct characteristics were observed among the PR-1 genes, with groups 1 and 2 being acidic with signal peptides, and group 3 being basic without signal peptides. All cloned PR-1 proteins belonged to the CAP superfamily (PF00188). Phylogenetic analysis revealed clustering patterns for acidic PR-1 proteins, and KEGG orthology showed associations with vital pathways, including MAPK signaling, plant hormone signal transduction, and plant-pathogen interaction. Secondary and tertiary structure analyses confirmed sequence conservation across studied species. Docking studies explored interactions between the cerato-platanin (CP) gene from Foc TR4 and Ma02_g15060 from banana, suggesting the potential hindrance of PR-1 antifungal activity through direct interaction.

conclusionsThe findings underscore the crucial role of cloned PR-1 genes in banana plant defense mechanisms against a broad spectrum of biotic stresses. These genes, especially those in groups 1 and 2, hold promise as candidates for developing stress-tolerant banana cultivars. The study provides valuable insights into the molecular aspects of banana defense strategies, emphasizing the potential applications of PR-1 genes in enhancing banana resilience.

Indexed as

FusariumMusaCloning, MolecularPhylogenyPlant DiseasesProtein Sorting SignalsProtein Sorting Signals3D structureBananaBiotic stressCerato-plataninGene expressionMolecular dockingPathogenesis related protein − 1

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PMID38403791
OpenAlexW4392143084

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