Evidence map›Paper›PMID 38403410›Full record

ArticleBiotechnology journal2024

Exploring engineering strategies that enhance de novo production of exotic cyclopropane fatty acids in Saccharomyces cerevisiae.

Wei Jiang, Huadong Peng, Lizhong He, Rodrigo Lesma-Amaro, Victoria S Haritos

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Wei JiangDepartment of Chemical and Biological Engineering, Monash University, Clayton, Victoria, Australia.
Huadong PengImperial College Centre for Synthetic Biology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0003-0635-0493
Lizhong HeDepartment of Chemical and Biological Engineering, Monash University, Clayton, Victoria, Australia.ORCID https://orcid.org/0000-0001-6569-526X
Rodrigo Lesma-AmaroImperial College Centre for Synthetic Biology, Imperial College London, London, UK.
Victoria S HaritosDepartment of Chemical and Biological Engineering, Monash University, Clayton, Victoria, Australia.ORCID https://orcid.org/0000-0003-4630-9647
Imperial College London · GBMonash University · AU

Funding

BBSRC BB/R01602X/1Monash Graduate Research International Travel AwardMonash Graduate ScholarshipMonash International Tuition Scholarships
6 · The paper itself

Abstract

Cycloalkanes have broad applications as specialty fuels, lubricants, and pharmaceuticals but are not currently available from renewable sources, whereas, production of microbial cycloalkanes such as cyclopropane fatty acids (CFA) has bottlenecks. Here, a systematic investigation was undertaken into the biosynthesis of CFA in Saccharomyces cerevisiae heterologously expressing bacterial CFA synthase. The enzyme catalyzes formation of a 3-membered ring in unsaturated fatty acids. Monounsaturated fatty acids in phospholipids (PL) are the site of CFA synthesis; precursor cis-Δ9 C16 and C18 fatty acids were enhanced through OLE1 and SAM2 overexpression which enhanced CFA in PL. CFA turnover from PL to storage in triacylglycerols (TAG) was achieved by phospholipase PBL2 overexpression and acyl-CoA synthase to increase flux to TAG. Consequently, CFA storage as TAG reached 12 mg g

Indexed as

CycloparaffinsSaccharomyces cerevisiaeCyclopropanesFatty AcidsPhospholipidsTriglyceridesCycloparaffinscyclopropane fatty acidsCyclopropanesFatty AcidsPhospholipidsTriglyceridesexotic fatty acidlipid metabolismmetabolic engineeringsynthetic biologytriacylglycerol

Identifiers

PMID38403410
PMCPMC11475713
OpenAlexW4392148011

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.