Evidence map›Paper›PMID 38402585›Full record

ArticleCell reports2024

Alternative mRNA splicing events and regulators in epidermal differentiation.

Shota Takashima, Wujianan Sun, Auke B C Otten, Pengfei Cai, Shaohong Isaac Peng, Elton Tong, Jolina Bui, McKenzie Mai, Oyumergen Amarbayar, Binbin Cheng and 4 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Splicing to orchestrate cell fate.Molecular therapy. Nucleic acids · 2025
    Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Shota TakashimaDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Wujianan SunDepartment of Oncology, The First Affiliated Hospital of USTC, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Auke B C OttenDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Pengfei CaiDepartment of Oncology, The First Affiliated Hospital of USTC, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Shaohong Isaac PengDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Elton TongDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Jolina BuiDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
McKenzie MaiDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Oyumergen AmarbayarDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Binbin ChengDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Rowen Jane OdangoDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA.
Zongkai LiDepartment of Oncology, The First Affiliated Hospital of USTC, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Kun QuDepartment of Oncology, The First Affiliated Hospital of USTC, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Bryan K SunDepartment of Dermatology, University of California San Diego, La Jolla, CA 92109, USA. Electronic address: sunb8@hs.uci.edu.
University of California San Diego · USUniversity of Science and Technology of China · CN

Funding

Insights to Noncoding Disease Variants using Mosaic DiseasesDP2HG012441 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUN, BRYAN · 2021 to 2024
$2.3M
Secreted Regulators of Epidermal HomeostasisK08AR067853 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUN, BRYAN · 2016 to 2020
$908k
NHGRI NIH HHS DP2 HG012441NIAMS NIH HHS K08 AR067853
6 · The paper itself

Abstract

Alternative splicing (AS) of messenger RNAs occurs in ∼95% of multi-exon human genes and generates diverse RNA and protein isoforms. We investigated AS events associated with human epidermal differentiation, a process crucial for skin function. We identified 6,413 AS events, primarily involving cassette exons. We also predicted 34 RNA-binding proteins (RBPs) regulating epidermal AS, including 19 previously undescribed candidate regulators. From these results, we identified FUS as an RBP that regulates the balance between keratinocyte proliferation and differentiation. Additionally, we characterized the function of a cassette exon AS event in MAP3K7, which encodes a kinase involved in cell signaling. We found that a switch from the short to long isoform of MAP3K7, triggered during differentiation, enforces the demarcation between proliferating basal progenitors and overlying differentiated strata. Our findings indicate that AS occurs extensively in the human epidermis and has critical roles in skin homeostasis.

Indexed as

Alternative SplicingRNA SplicingExonsHumansProtein IsoformsRNA, MessengerProtein IsoformsRNA, Messengeralternative splicingCP: Molecular biologyCP: Stem cell researchepidermal differentiationMAP3K7skinskin homeostasis

Identifiers

PMID38402585
PMCPMC11293371
OpenAlexW4392119594

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.