ArticleScientific reports2024
Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06560216 (Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm), which is not on this map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm
Who cites it
14 citing papers in PubMed.
- Mitochondria-targeted SkQ1 supplementation enhances post-thaw quality, cryosurvival, and functional competence of rooster spermatozoa.Poultry science · 2026Article
- Synergistic protection of chilled canine spermatozoa by apigenin and N-acetylcysteine amide through enhanced antioxidant defense and mitochondrial function.BMC veterinary research · 2026Article
- Protective Effects of Fisetin Against Oxidative Stress in Human Sperm: Implications for Cryopreservation.Antioxidants (Basel, Switzerland) · 2026Article
- Effect of nanoliposomal gamma-oryzanol on cryosurvival, oxidative balance, and fertility of frozen-thawed ram spermatozoa.Scientific reports · 2026Article
- Comparative Analysis of Gut Microbiota and Metabolome of Captive Male Malayan Pangolins with Normal and Abnormal Reproduction.Animals : an open access journal from MDPI · 2026Article
- Influence of Phlai (Molecules (Basel, Switzerland) · 2026Article
- Type of Extender and Equilibration Time as Factors Affecting Post-Thaw Quality Characteristics of Turkey Semen.Animals : an open access journal from MDPI · 2025Article
- Optimization of Duck Semen Freezing Procedure and Regulation of Oxidative Stress.Animals : an open access journal from MDPI · 2025Article
- Mitochondrial specific antioxidant MitoPBN mitigates oxidative stress and improves mitochondrial function in cryopreserved ram sperm.Scientific reports · 2025Article
- Comparative assessment of thawing methods for frozen rooster sperm.Frontiers in veterinary science · 2025Article
- The Potential Effect of Bualuang (WhiteLife (Basel, Switzerland) · 2024Article
- Anacyclus pyrethrum enhances fertility in cadmium-intoxicated male rats by improving sperm functions.BMC complementary medicine and therapies · 2024Article
- Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine.Antioxidants (Basel, Switzerland) · 2024Article
- Apigenin as a Promising Agent for Enhancing Female Reproductive Function and Treating Associated Disorders.Biomedicines · 2024Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This pioneering research investigated apigenin potential to augment rooster sperm cryosurvival in an extender model. Apigenin is a natural antioxidant flavonoid showing promise for improved post-thaw sperm function. However, its effects on avian semen cryopreservation remain unexplored. This first study supplemented rooster sperm Lake extender with 0, 50, 100, 200, 400 μmol/L apigenin to determine the optimal concentrations for post-thaw quality. Supplementation with 100 μmol/L apigenin resulted in significant enhancements in total motility (from 41.5% up to 71.5%), progressive motility (18.1% to 29.1%) (p < 0.05), membrane integrity (40% to 68%), mitochondrial function (p < 0.001), viability (37% to 62%) and total antioxidant capacity (p < 0.001) compared to the control. It also substantially reduced percentages of abnormal morphology, reactive oxygen species and apoptosis (p < 0.001). Although 200 μmol/L apigenin significantly enhanced some attributes, effects were markedly lower than 100 μmol/L. Higher doses did not improve cryoprotective parameters. This indicates 100 μmol/L as the optimal apigenin concentration. This represents the first report of apigenin protecting rooster sperm from cryodamage. The natural antioxidant improved post-thaw sperm quality, likely by suppressing oxidative stress and apoptosis. Apigenin shows promise for enhancing rooster sperm cryosurvival.
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