Evidence map›Paper›PMID 38402195›Full record

ArticleMicrobial cell factories2024

Unlocking Nature's Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter.

Neetu Rajak, Trishna Dey, Yash Sharma, Vedanth Bellad, Pundi N Rangarajan

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Metabolic engineering ofApplied and environmental microbiology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Neetu RajakDepartment of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
Trishna DeyDepartment of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
Yash SharmaDepartment of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
Vedanth BelladDepartment of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
Pundi N RangarajanDepartment of Biochemistry, Indian Institute of Science, Bangalore, 560012, India. pnr@iisc.ac.in.
Indian Institute of Science Bangalore · IN

Funding

Biotechnology Industry Research Assistance Council BT/AIR01307/PACE-22-20Department of Science and Technology, Ministry of Science and Technology, India Fund for Improvement of Science and Technology InfrastructureHuman Resource Development Centre, Council of Scientific And Industrial Research JRF/SRFScience and Engineering Research Board CRG/2022/000598Science and Engineering Research Board JCB/2022/000013Science and Engineering Research Board TTR/2022/00023
6 · The paper itself

Abstract

backgroundKomagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase (PEPCK) is induced by glutamate. Glutamate-inducible synthesis of these enzymes is regulated by Rtg1p, a cytosolic, basic helix-loop-helix protein. Here, we report food-grade monosodium glutamate (MSG)-inducible recombinant protein production from K. phaffii PEPCK promoter (P

resultsP

conclusionsWe have designed an induction medium for recombinant protein production from K. phaffii P

Indexed as

MethanolSaccharomycetalesEthanolGlutamatesPichiaRecombinant ProteinsSodium GlutamateEthanolGlutamatesMethanolRecombinant ProteinsSodium GlutamateGlutamate inducible expression systemKomagataella phaffiiMonosodium glutamatePEPCK promoter

Identifiers

PMID38402195
PMCPMC10893637
OpenAlexW4392131549

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.