Laser-Induced Intracellular Delivery: Exploiting Gold-Coated Spiky Polymeric Nanoparticles and Gold Nanorods under Near-Infrared Pulses for Single-Cell Nano-Photon-Poration.

Ashish Kumar, Bishal Kumar Nahak, Pallavi Gupta, Tuhin Subhra Santra, Fan-Gang Tseng

Open access · goldAbstract read

In one paragraph

Article in Micromachines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

1.5field-weighted citation impact, top 21% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

Unraveling Plasmon-Enhanced Reactive Oxygen Species Generation through Ultrafast Light.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Ashish KumarDepartment of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.ORCID 0000-0002-7855-1756

Bishal Kumar NahakDepartment of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.

Pallavi GuptaDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, India.

Tuhin Subhra SantraDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, India.ORCID 0000-0002-9403-2155

Fan-Gang TsengDepartment of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.ORCID 0000-0001-7654-6905

National Tsing Hua University · TWIndian Institute of Technology Madras · IN

Funding

DBT/Wellcome Trust India Alliance Fellowship IA/E/16/1/503062Frontier Research Center on Fundamental and Applied Sciences of Matters, from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education MOST 111-2634-F-007-007, MOST 110-2634-F-007-023, and MOE111QR001I5National Health Research Institutes NHRI-EX112-11126BIVeterans General Hospital and University System of the Taiwan Joint Research Program (VGHUST) ( VGHUST109-V6-1-1 and VGHUST111-G5-1-1

6 · The paper itself

Abstract

This study explores the potential of laser-induced nano-photon-poration as a non-invasive technique for the intracellular delivery of micro/macromolecules at the single-cell level. This research proposes the utilization of gold-coated spiky polymeric nanoparticles (Au-PNPs) and gold nanorods (GNRs) to achieve efficient intracellular micro/macromolecule delivery at the single-cell level. By shifting the operating wavelength towards the near-infrared (NIR) range, the intracellular delivery efficiency and viability of Au-PNP-mediated photon-poration are compared to those using GNR-mediated intracellular delivery. Employing Au-PNPs as mediators in conjunction with nanosecond-pulsed lasers, a highly efficient intracellular delivery, while preserving high cell viability, is demonstrated. Laser pulses directed at Au-PNPs generate over a hundred hot spots per particle through plasmon resonance, facilitating the formation of photothermal vapor nanobubbles (PVNBs). These PVNBs create transient pores, enabling the gentle transfer of cargo from the extracellular to the intracellular milieu, without inducing deleterious effects in the cells. The optimization of wavelengths in the NIR region, coupled with low laser fluence (27 mJ/cm

Indexed as

Au-PNPgold nanorodsintracellular deliveryLSPRnanosecond-pulsed laserphoton-porationPVNB

Identifiers

PMID38398898

DOI10.3390/mi15020168

PMCPMC10890628

OpenAlexW4391141310

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.