ArticleGenes2024
Exploring the Impact of Primer-Template Mismatches on PCR Performance of DNA Polymerases Varying in Proofreading Activity.
Article in Genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
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- A Multiplex PCR-Based Assay for Authentication of Six Commercially Important Cephalopod Species.Foods (Basel, Switzerland) · 2026Article
- Accurate and sensitive TaqMan real-time PCR assays to detect and quantify simian malaria parasites Plasmodium cynomolgi, Plasmodium inui and Plasmodium coatneyi.Malaria journal · 2026Article
- Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay.Microbiology spectrum · 2026Article
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- Application of SNV Detection Methods for Market Control of Food Products from New Genomic Techniques.International journal of molecular sciences · 2026Review
- Improved characterisation of coral-associated fungal communities using host DNA depletion and a novel ITS primer.ISME communications · 2026Article
- A multiplex PCR for rapid detection of vancomycin and linezolid resistance genes in clinical and environmental enterococci: A one health diagnostic approach.One health (Amsterdam, Netherlands) · 2025Article
- Development of a Multiplex PCR Assay for Simultaneous Identification of Six Commercially Important Bivalves.Foods (Basel, Switzerland) · 2025Article
- Comparative evaluation of three polymerase chain reaction primer sets for accurate molecular detection ofVeterinary world · 2025Article
- Artificial base mismatches-mediated PCR (ABM-PCR) for detecting clinically relevant single-base mutations.Clinical chemistry and laboratory medicine · 2025Article
- Mitigating drought stress and enhancing maize resistance through biopriming with Rhizopus arrhizus: insights into Morpho-Biochemical and molecular adjustments.BMC plant biology · 2025Article
- Development of a Highly Multiplexed RT-LAMP Assay for Coverage of Genetic Sequence Diversity.Analytical chemistry · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase chain reaction (PCR) is a widely used technique in gene expression analysis, diagnostics, and various molecular biology applications. However, the accuracy and sensitivity of PCR can be compromised by primer-template mismatches, potentially leading to erroneous results. In this study, we strategically designed 111 primer-template combinations with varying numbers, types, and locations of mismatches to meticulously assess their impact on qPCR performance while two distinctly different types of DNA polymerases were used. Notably, when a single-nucleotide mismatch occurred at the 3' end of the primer, we observed significant decreases in the analytical sensitivity (0-4%) with Invitrogen™ Platinum™
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.