Evidence map›Paper›PMID 38397141›Full record

ArticleGenes2024

Validation of Endogenous Control Genes by Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction for Acute Leukemia Gene Expression Studies.

Flávia Melo Cunha de Pinho Pessoa, Vitória Beatriz de Jesus Viana, Marcelo Braga de Oliveira, Beatriz Maria Dias Nogueira, Rodrigo Monteiro Ribeiro, Deivide de Sousa Oliveira, Germison Silva Lopes, Ricardo Parente Garcia Vieira, Manoel Odorico de Moraes Filho, Maria Elisabete Amaral de Moraes and 3 more

Abstract read
In one paragraph

Article in Genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Flávia Melo Cunha de Pinho PessoaDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.
Vitória Beatriz de Jesus VianaDepartment of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém 66073-005, PA, Brazil.
Marcelo Braga de OliveiraDepartment of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém 66073-005, PA, Brazil.
Beatriz Maria Dias NogueiraDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.
Rodrigo Monteiro RibeiroDepartment of Hematology, Fortaleza General Hospital (HGF), Fortaleza 60150-160, CE, Brazil.
Deivide de Sousa OliveiraDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.
Germison Silva LopesDepartment of Hematology, César Cals General Hospital, Fortaleza 60015-152, CE, Brazil.ORCID 0000-0002-7125-5620
Ricardo Parente Garcia VieiraDepartment of Hematology, São Vicente de Paulo Maternity Hospital, Barbalha 63180-000, CE, Brazil.
Manoel Odorico de Moraes FilhoDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.
Maria Elisabete Amaral de MoraesDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.
André Salim KhayatDepartment of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém 66073-005, PA, Brazil.ORCID 0000-0002-3451-6369
Fabiano Cordeiro MoreiraDepartment of Biological Sciences, Oncology Research Center, Federal University of Pará, Belém 66073-005, PA, Brazil.ORCID 0000-0002-2799-3546
Caroline Aquino Moreira-NunesDepartment of Medicine, Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza 60430-275, CE, Brazil.ORCID 0000-0001-5845-3481

Funding

Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico PPSUS
6 · The paper itself

Abstract

Reference genes are used as internal reaction controls for gene expression analysis, and for this reason, they are considered reliable and must meet several important criteria. In view of the absence of studies regarding the best reference gene for the analysis of acute leukemia patients, a panel of genes commonly used as endogenous controls was selected from the literature for stability analysis: Glyceraldehyde-3-phosphate dehydrogenase (

Indexed as

Gene Expression ProfilingLeukemiaAcute DiseaseAnimalsGene ExpressionGenes, EssentialGlyceraldehyde-3-Phosphate DehydrogenasesHumansMiceReverse Transcriptase Polymerase Chain ReactionGlyceraldehyde-3-Phosphate Dehydrogenasesgene expressionleukemiareference genesRT-qPCR

Identifiers

PMID38397141
PMCPMC10887733

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.