ArticleDiagnostic pathology2024
Quantitative assessment of H&E staining for pathology: development and clinical evaluation of a novel system.
Article in Diagnostic pathology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Current quality challenges in H&E preparation: The critical foundation for increased use of digital pathology and AI emergence.Virchows Archiv : an international journal of pathology · 2026Article
- Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Green Biosynthesis of Bioactive Streptomyces Metabolites Using Edible Mushrooms: Implications for Glycemic Control and Hepatic Protection in Diabetic Rats.Food science & nutrition · 2026Article
- Functional cytokine-based classification of tumor-infiltrating lymphocytes in melanoma.Scientific reports · 2026Article
- AI and the digital pathology revolution: clinical applications in cancer diagnosis and assessment.Expert review of molecular diagnostics · 2026Review
- Improved hemodynamic and cardiorespiratory instability with vitamin D supplementation in an obstructive sleep apnea rat models.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2026Article
- Staining normalization in histopathology: Method benchmarking using multicenter dataset.Scientific reports · 2026Article
- StainStyleSampler: clustering-based sampling of whole slide image appearances.Diagnostic pathology · 2026Article
- Development of a machine learning model for preoperative prediction of spread through air spaces in resectable non-small cell lung cancer: A single-center retrospective study.Oncology letters · 2026Article
- Astragaloside IV Alleviates Lupus Nephritis by Inhibiting Podocyte Ferroptosis via the PI3K/AKT/Nrf2 Pathway.Drug design, development and therapy · 2026Article
- Dose-dependent vitamin D3 supplementation attenuates endothelial dysfunction and renal injury in an obstructive sleep apnea rat model.Frontiers in pharmacology · 2026Article
- DenseUNet for Breast Cancer Segmentation in Histopathological Images.Journal of medical signals and sensors · 2026Article
- Application of artificial intelligence in hepatology.Frontiers in digital health · 2026Review
- A Lightweight Cross-Gated Dual-Branch Attention Network for Colon and Lung Cancer Diagnosis from Histopathological Images.Medical sciences (Basel, Switzerland) · 2025Article
- Development of a Novel Aluminium Chloride-Induced Zebrafish Model of Alzheimer's Disease: Involvement of Oxidative Stress, Cholinergic Dysfunction, and Gut Pathophysiology.Neurotoxicity research · 2025Article
- Role of Mesenchymal Stem Cell Exosomes and Injectable Platelet Rich Fibrin on Structure and Function of Submandibular Salivary Gland of Aged Albino Rats.The Saudi dental journal · 2025Article
- Pathologist-interpretable breast cancer subtyping and stratification from AI-inferred nuclear features.bioRxiv : the preprint server for biology · 2025Article
- Albumin Reduces Hepatic Steatosis and Inflammation in High-Fat-Diet-Fed Mice.International journal of molecular sciences · 2025Article
- Whole-body inhalation study of nanoparticle-enhanced vegetable oil metalworking fluids in mice for assessing occupational health risks.Scientific reports · 2025Article
- Automated cell properties toolbox from 3D bioprinted hydrogel scaffolds via deep learning and optical coherence tomography.Biomedical optics express · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaining tissue samples to visualise cellular detail and tissue structure is at the core of pathology diagnosis, but variations in staining can result in significantly different appearances of the tissue sample. While the human visual system is adept at compensating for stain variation, with the growth of digital imaging in pathology, the impact of this variation can be more profound. Despite the ubiquity of haematoxylin and eosin staining in clinical practice worldwide, objective quantification is not yet available. We propose a method for quantitative haematoxylin and eosin stain assessment to facilitate quality assurance of histopathology staining, enabling truly quantitative quality control and improved standardisation.
methodsThe stain quantification method comprises conventional microscope slides with a stain-responsive biopolymer film affixed to one side, called stain assessment slides. The stain assessment slides were characterised with haematoxylin and eosin, and implemented in one clinical laboratory to quantify variation levels.
resultsStain assessment slide stain uptake increased linearly with duration of haematoxylin and eosin staining (r = 0.99), and demonstrated linearly comparable staining to samples of human liver tissue (r values 0.98-0.99). Laboratory implementation of this technique quantified intra- and inter-instrument variation of staining instruments at one point in time and across a five-day period.
conclusionThe proposed method has been shown to reliably quantify stain uptake, providing an effective laboratory quality control method for stain variation. This is especially important for whole slide imaging and the future development of artificial intelligence in digital pathology.
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