Evidence map›Paper›PMID 38393603›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Quantitative Analysis of Whole-Mount Fluorescence-Stained Tumor Spheroids in Phenotypic Drug Screens.

Elina Nuernberg, Roman Bruch, Mathias Hafner, Ruediger Rudolf, Mario Vitacolonna

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Elina NuernbergInstitute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany.
Roman BruchInstitute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany.
Mathias HafnerInstitute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany.
Ruediger RudolfInstitute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany.
Mario VitacolonnaInstitute of Molecular and Cell Biology, Mannheim University of Applied Sciences, Mannheim, Germany. m.vitacolonna@hs-mannheim.de.
Mannheim University of Applied Sciences · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional cell cultures, such as spheroids or organoids, serve as important models for drug screening purposes. Optical tissue clearing (OTC) enhances the visualization of fluorescence stainings and enables in toto microscopy of 3D cell culture models. Furthermore, subsequent automated image analysis tools convert qualitative confocal image sets into quantitative data. In this chapter, we describe a detailed protocol for preparation of HT29 cancer spheroids, 3D in toto immunostaining, glycerol-based OTC, whole-mount imaging, and semi-automated downstream image processing and segmentation for nuclear image analysis using open-source software.

Indexed as

NeoplasmsQuinolinium CompoundsSpheroids, CellularThiazolesHumansImage Processing, Computer-AssistedSoftware1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene)quinoliniumQuinolinium CompoundsThiazoles3D cell cultureImage analysisOpen sourceOptical tissue clearingOrganoidsSegmentationSpheroid

Identifiers

PMID38393603
OpenAlexW4392103237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.