Evidence map›Paper›PMID 38393593›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland.

Alison Kuony, Matea Brezak, René-Marc Mège, Zuzana Sumbalova Koledova

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alison KuonyUniversité Paris Cité, CNRS, Institut Jacques Monod, Paris, France. alison.kuony@ijm.fr.ORCID 0000-0001-9132-4921
Matea BrezakDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.ORCID 0000-0001-8382-4782
René-Marc MègeUniversité Paris Cité, CNRS, Institut Jacques Monod, Paris, France.ORCID 0000-0001-8128-5543
Zuzana Sumbalova KoledovaDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.ORCID 0000-0002-9333-1399

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands.

Indexed as

Lacrimal ApparatusAnimalsCell Culture Techniques, Three DimensionalEpitheliumMiceMorphogenesisOrgan Culture Techniques3D cultureBranchingDevelopmentEpitheliumLacrimal glandMorphogenesis

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.