Evidence map›Paper›PMID 38393471›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Measuring Molecular Diffusion in Self-Organizing Xenopus Extracts by Fluorescence Correlation Spectroscopy.

William Y C Huang, James E Ferrell, Xianrui Cheng

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

William Y C HuangDepartment of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0002-6061-8887
James E FerrellDepartment of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0003-4767-3926
Xianrui ChengDepartment of Biological Sciences, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0002-5258-7590
Stanford University · USUniversity of Southern California · US

Funding

Cytoplasmic organization and systems-level function in Xenopus extractsR35GM131792 · NIGMS · STANFORD UNIVERSITY · PI JAMES E. FERRELL · 2019 to 2026
$5.6M
Reconstructing and deconstructing intracellular signaling at the membrane-cytosol interfaceK99GM143481 · NIGMS · STANFORD UNIVERSITY · PI HUANG, YUAN-CHI · 2022 to 2023
$200k
NIGMS NIH HHS K99 GM143481NIGMS NIH HHS R35 GM131792
6 · The paper itself

Abstract

The cytoplasm is densely packed with macromolecules and organelles, displaying viscoelastic properties at various scales. How biochemical reactions function efficiently enough in a seemingly jammed environment remains elusive. Cell-free Xenopus laevis extracts represent a powerful system for investigating the biochemistry and biophysics of living systems. Here we present a protocol for characterizing macromolecular diffusion in self-organizing cytoplasmic extracts using fluorescence correlation spectroscopy (FCS), which measures the motions on a distance scale of ~200 nm. The method can also be used to characterize diffusion in the cytoplasm as it progresses through different phases of the cell cycle.

Indexed as

Xenopus laevisAnimalsCell DivisionCytoplasmCytosolDiffusionSpectrometry, FluorescenceSpectrum AnalysisCell extractsCytoplasmDiffusionFluorescence correlation spectroscopySelf-organization

Identifiers

PMID38393471
OpenAlexW4392106349

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.