Evidence map›Paper›PMID 38393469›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Dissecting the Multiple Functions of the Polo-Like Kinase 1 in the C. elegans Zygote.

Griselda Velez-Aguilera, Batool Ossareh-Nazari, Lionel Pintard

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
4.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Griselda Velez-AguileraUniversité Paris Cité, CNRS, Institut Jacques Monod, Paris, France.
Batool Ossareh-NazariUniversité Paris Cité, CNRS, Institut Jacques Monod, Paris, France.
Lionel PintardUniversité Paris Cité, CNRS, Institut Jacques Monod, Paris, France. lionel.pintard@ijm.fr.
Centre National de la Recherche Scientifique · FRLa Ligue Contre le Cancer · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plk1 (polo-like kinase 1) is an evolutionarily conserved serine/threonine kinase instrumental for mitotic entry and progression. Beyond these canonical functions, Plk1 also regulates cell polarization and cell fate during asymmetric cell divisions in C. elegans and D. melanogaster. Plk1 contains a specialized phosphoserine-threonine binding domain, the polo-box domain (PBD), which localizes and concentrates the kinase at its various sites of action within the cell in space and time. Here we present protocols to express and purify the C. elegans Plk1 kinase along with biochemical and phosphoproteomic approaches to interrogate the PBD interactome and to dissect Plk1 substrate interactions. These protocols are most suitable for the identification of Plk1 targets in C. elegans embryos but can be easily adapted to identify and study Plk1 substrates from any source."

Indexed as

Caenorhabditis elegansCell Cycle ProteinsAnimalsDrosophila melanogasterPolo-Like Kinase 1Protein BindingProtein Kinase InhibitorsZygoteCell Cycle ProteinsPolo-Like Kinase 1Protein Kinase InhibitorsC. elegans embryosPhosphoproteomicsPolo-box domainPolo-like kinase 1

Identifiers

PMID38393469
OpenAlexW4392105946

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.