Studying Translesion DNA Synthesis Using Xenopus In Vitro Systems.

Antoine Aze, James R A Hutchins, Domenico Maiorano

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 95% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Antoine AzeGenome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France.

James R A HutchinsGenome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France.

Domenico MaioranoGenome Surveillance and Stability Laboratory, Institute of Human Genetics, UMR9002, CNRS-University of Montpellier, Montpellier, France. domenico.maiorano@igh.cnrs.fr.

Centre National de la Recherche Scientifique · FRUniversité de Montpellier · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free extracts derived from Xenopus eggs have been widely used to decipher molecular pathways involved in several cellular processes including DNA synthesis, the DNA damage response, and genome integrity maintenance. We set out assays using Xenopus cell-free extracts to study translesion DNA synthesis (TLS), a branch of the DNA damage tolerance pathway that allows replication of damaged DNA. Using this system, we were able to recapitulate TLS activities that occur naturally in vivo during early embryogenesis. This chapter describes protocols to detect chromatin-bound TLS factors by western blotting and immunofluorescence microscopy upon induction of DNA damage by UV irradiation, monitor TLS-dependent mutagenesis, and perform proteomic screening.

Indexed as

ProteomicsTranslesion DNA SynthesisAnimalsDNADNA DamageDNA RepairDNA ReplicationXenopus laevisDNACell-free extractChromatinDNA damage toleranceDNA replicationMutagenesisProteomicsXenopus laevis

Identifiers

PMID38393467

DOI10.1038/nature19840

OpenAlexW4392105995

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.