Evidence map›Paper›PMID 38393428›Full record

ArticleMolecular biology reports2024

Regulation of blue infertile flower pigmentation by WD40 transcription factor HmWDR68 in Hydrangea macrophylla 'forever summer'.

Jingyi Gong, Yu Wang, Chao Xue, Linshi Wu, Song Sheng, Meng Wang, Jiqing Peng, Shoujin Cao

Abstract read
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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Virus-Induced Gene Silencing (VIGS) inPlants (Basel, Switzerland) · 2024
    Article
  6. Genome-Wide Identification and Analysis of WD40 Family and Its Expression inInternational journal of molecular sciences · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Jingyi Gong *College of Life Science and Technology, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China.
Yu Wang *College of Forestry, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China.
Chao XueCollege of Life Science and Technology, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China.
Linshi WuHunan Botanical Garden, Changsha, Hunan, 410000, China.
Song ShengCollege of Forestry, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China.
Meng WangCollege of Forestry, Southwest Forestry University, Kunming, Yunnan, 650000, China.
Jiqing PengCollege of Life Science and Technology, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China. pengjiqing17@csuft.edu.cn.
Shoujin CaoCollege of Forestry, Central South University of Forestry & Technology, Changsha, Hunan, 410004, China. 1398524816@qq.com.
Central South University · CNCentral South University of Forestry and Technology · CNHunan Normal University · CNSouthwest Forestry University · CN

Funding

Central Finance Forestry Science and Technology Promotion Demonstration Fund Project [2022] XT16Scientific Research Project of Hunan Provincial Department of Education 22B0261
6 · The paper itself

Abstract

backgroundWD40 transcription factors are crucial in plant growth and developmental, significantly impacting plant growth regulation. This study investigates the WD40 transcription factor HmWDR68's role in developing the distinctive blue infertile flower colors in Hydrangea macrophylla 'Forever Summer'. METHODS AND

resultsThe HmWDR68 gene was isolated by PCR, revealing an open reading frame of 1026 base pairs, which encodes 341 amino acids. Characterized by four WD40 motifs, HmWDR68 is a member of the WD40 family. Phylogenetic analysis indicates that HmWDR68 shares high homology with PsWD40 in Camellia sinensis and CsWD40 in Paeonia suffruticosa, both of which are integral in anthocyanin synthesis regulation. Quantitative real-time PCR (qRT-PCR) analysis demonstrated that HmWDR68 expression in the blue infertile flowers of 'Forever Summer' hydrangea was significantly higher compared to other tissues and organs. Additionally, in various hydrangea varieties with differently colored infertile flowers, HmWDR68 expression was markedly elevated in comparison to other hydrangea varieties, correlating with the development of blue infertile flowers. Pearson correlation analysis revealed a significant association between HmWDR68 expression and the concentration of delphinidin 3-O-glucoside, as well as key genes involved in anthocyanin biosynthesis (HmF3H, HmC3'5'H, HmDFR, and HmANS) in the blue infertile flowers of 'Forever Summer' hydrangea (P < 0.01).

conclusionThese findings suggest HmWDR68 may specifically regulate blue infertile flower formation in hydrangea by enhancing delphinidin-3-O-glucoside synthesis, modulating expression of HmF3H, HmC3'5'H, HmDFR and HmANS. This study provides insights into HmWDR68's role in hydrangea's blue flowers development, offering a foundation for further research in this field.

Indexed as

AnthocyaninsHydrangeaFlowersGene Expression Regulation, PlantGlucosidesPhylogenyPigmentationTranscription FactorsAnthocyaninsGlucosidesTranscription FactorsExpression patternFlower colorGene cloningHydrangea macrophyllaTranscription factor

Identifiers

PMID38393428
OpenAlexW4392126030

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.