ArticleScientific reports2024
Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 10 citations in OpenAlex.
- High-throughput in situ single particle X-ray imaging of dehydrating viral capsids.Light, science & applications · 2026Article
- Native mass spectrometry meets X-rays for the elucidation of protein structures.Biochemical Society transactions · 2026Review
- Partial orientation retrieval of proteins from X-ray free-electron-laser induced explosions for single particle imaging.Scientific reports · 2025Article
- Impact of gas background on XFEL single-particle imaging.Scientific reports · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.Journal of synchrotron radiation · 2025Article
- Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction.Journal of applied crystallography · 2024Article
- High-Performance Molecular Dynamics Simulations for Native Mass Spectrometry of Large Protein Complexes with the Fast Multipole Method.Analytical chemistry · 2024Article
- Conditional guided generative diffusion for particle accelerator beam diagnostics.Scientific reports · 2024Article
- Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers.Structural dynamics (Melville, N.Y.) · 2024Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated proteins are limited by three factors: low signal strength, limited data and high background from gas scattering. The last two factors are largely due to the shortcomings of the aerosol sample delivery methods in use. Here we present our modified electrospray ionization (ESI) source, which we dubbed helium-ESI (He-ESI). With it, we increased particle delivery into the interaction region by a factor of 10, for 26 nm-sized biological particles, and decreased the gas load in the interaction chamber corresponding to an 80% reduction in gas scattering when compared to the original ESI. These improvements have the potential to significantly increase the quality and quantity of SPI diffraction patterns in future experiments using He-ESI, resulting in higher-resolution structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.