Evidence map›Paper›PMID 38386140›Full record

ArticleApplied biochemistry and biotechnology2024

Efficient Expression and Characterization of an Endo-Type Lyase HCLase_M28 and Its Gradual Scale-Up Fermentation for the Preparation of Chondroitin Sulfate Oligosaccharides.

Ruibao Ju, Baoqin Han, Feng Han, Yanfei Peng

Abstract read
PubMed Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. A novelFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ruibao JuCollege of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Baoqin HanCollege of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Feng HanSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China.
Yanfei PengCollege of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. yanfeipeng@ouc.edu.cn.
Ocean University of China · CNQingdao National Laboratory for Marine Science and Technology · CN

Funding

National Key R&D Program of China 2018YFC0311105Natural Science Foundation of Shandong Province on the Key Basic Research Project ZR2022ZD21
6 · The paper itself

Abstract

Glycosaminoglycan (GAG) lyases have been critical in structural and functional studies of GAGs. HCLase_M28, a lyase identified from the genome of Microbacterium sp. M28 was heterologously expressed, enzymatically characterized, and prepared in large-scale fermentation for the production of chondroitin sulfate (CS) oligosaccharides. Results showed that the expression of HCLase_M28 in Escherichia coli BL21 (DE3)-pET24a-HCLase_M28

Indexed as

Chondroitin SulfatesEscherichia coliFermentationOligosaccharidesBacillus subtilisBacterial ProteinsGene ExpressionPolysaccharide-LyasesBacterial ProteinsChondroitin SulfatesOligosaccharidesPolysaccharide-LyasesGlycosaminoglycan lyaseHeterologous expressionLarge-scale fermentationOligosaccharides

Identifiers

PMID38386140
OpenAlexW4392057628

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.