Evidence map›Paper›PMID 38385979›Full record

ArticleAging2024

MicroRNA-320a enhances LRWD1 expression through the AGO2/FXR1-dependent pathway to affect cell behaviors and the oxidative stress response in human testicular embryonic carcinoma cells.

Yi-Hsiung Lin, Chia-Hui Su, Hsin-Mei Chen, Ming-Syuan Wu, Hsien-An Pan, Chia-Ning Chang, Yu-Sheng Cheng, Wen-Tsan Chang, Chien-Chih Chiu, Yen-Ni Teng

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In one paragraph

Article in Aging, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.2field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Yi-Hsiung LinCenter for Lipid Biosciences, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Chia-Hui SuDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 700, Taiwan.
Hsin-Mei ChenDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 700, Taiwan.
Ming-Syuan WuDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 700, Taiwan.
Hsien-An PanAn-An Women and Children Clinic, Tainan 704, Taiwan.
Chia-Ning ChangDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 700, Taiwan.
Yu-Sheng ChengDepartment of Urology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Wen-Tsan ChangDepartment of Surgery, Division of General and Digestive Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
Chien-Chih ChiuDepartment of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Yen-Ni TengDepartment of Biological Sciences and Technology, National University of Tainan, Tainan 700, Taiwan.
National University of Tainan · TWKaohsiung Medical University · TWAn-Nan Hospital · TWNational Cheng Kung University Hospital · TWNational Sun Yat-sen University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTesticular cancer is fairly rare but can affect fertility in adult males. Leucine-rich repeats- and WD repeat domain-containing protein 1 (LRWD1) is a sperm-specific marker that mainly affects sperm motility in reproduction. Our previous study demonstrated the impact of LRWD1 on testicular cancer development; however, the underlying mechanisms remain unclear.

methodsIn this study, various plasmids associated with LRWD1 and miR-320a manipulation were used to explore the roles and regulatory effects of these molecules in NT2D1 cellular processes. A Dual-Glo luciferin-luciferase system was used to investigate LRWD1 transcriptional activity, and qRT-PCR and western blotting were used to determine gene and protein expression.

resultsThe results suggested that miR-320a positively regulated LRWD1 and positively correlated with NT2D1 cell proliferation but negatively correlated with cell migration and invasion ability. In addition, the miRNA-ribonucleoprotein complex AGO2/FXR1 was shown to be essential in the mechanism by which miR-320a regulates LRWD1 mRNA expression. As miR-320a was required to regulate LRWD1 expression through the AGO2 and FXR1 complex, eEF2 and eLF4E were also found to be involved in miR-320a increasing LRWD1 expression. Furthermore, miR-320a and LRWD1 were responsive to oxidative stress, and NRF2 was affected by the presence of miR-320a in response to ROS stimulation.

conclusionsThis is the first study showing the role of miR-320a in upregulating the testicular cancer-specific regulator LRWD1 and the importance of the AGO2/FXR1 complex in miR-320a-mediated upregulation of LRWD1 during testicular cancer progression.

Indexed as

CarcinomaMicroRNAsNeoplasms, Germ Cell and EmbryonalTesticular NeoplasmsCell Line, TumorCell ProliferationHumansMaleMicrotubule ProteinsOxidative StressRNA-Binding ProteinsSemenSperm MotilityTranscription FactorsFXR1 protein, humanLRWD1 protein, humanMicroRNAsMicrotubule ProteinsMIRN320A microRNA, humanRNA-Binding ProteinsTranscription FactorsAGO2FXR1LRWD1miR-320atesticular cancer

Identifiers

PMID38385979
PMCPMC10929808
OpenAlexW4391996952

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.