Evidence map›Paper›PMID 38384793›Full record

ArticleMaterials today. Bio2024

Multiphoton lithography with protein photoresists.

Dmitry Sivun, Eljesa Murtezi, Tina Karimian, Kurt Hurab, Maryam Marefat, Elena Klimareva, Christoph Naderer, Boris Buchroithner, Thomas A Klar, Georgii Gvindzhiliia and 2 more

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In one paragraph

Article in Materials today. Bio, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Dmitry SivunDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Eljesa MurteziDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Tina KarimianDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Kurt HurabDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Maryam MarefatDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Elena KlimarevaDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Christoph NadererDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Boris BuchroithnerDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.
Thomas A KlarInstitute of Applied Physics, Johannes Kepler University Linz, Altenberger Straße 69, 4040, Linz, Austria.
Georgii GvindzhiliiaInstitute of Applied Physics, Johannes Kepler University Linz, Altenberger Straße 69, 4040, Linz, Austria.
Andreas HornerInstitute of Biophysics, Johannes Kepler University Linz, Gruberstraße 40, 4020, Linz, Austria.
Jaroslaw JacakDepartment of Medical Engineering, University of Applied Sciences Upper Austria, Garnisonstraße 21, 4020, Linz, Austria.

Funding

Austrian Science Fund FWF P 26461
6 · The paper itself

Abstract

Recently, 2D/3D direct laser writing has attracted increased attention due to its broad applications ranging from biomedical engineering to aerospace. 3D nanolithography of water-soluble protein-based scaffolds have been envisioned to provide a variety of tunable properties. In this paper, we present a functional protein-based photoresist with tunable mechanical properties that is suitable for multiphoton lithography (MPL). Through the use of methacrylated streptavidin or methacrylated bovine serum albumin in combination with polyethylene glycol diacrylate or methacrylated hyaluronic acid as crosslinkers and a vitamin-based photoinitiator, we were able to write two- and three-dimensional structures as small as 200 nm/600 nm lateral/axial features, respectively. We also demonstrated that Young's modulus can be tuned by the photoresist composition, and we were able to achieve values as low as 40 kPa. Furthermore, we showed that Young's modulus can be recovered after drying and rehydration (i.e. shelf time determination). The retained biological functionality of the streptavidin scaffolds was demonstrated using fluorescently labelled biotins. Using single-molecule fluorescence microscopy, we estimated the density of streptavidin in the written features (1.8 ± 0.2 × 10

Indexed as

Extracellular vesicleFunctional photoresistMultiphoton lithographyProtein printingTissue scaffolds

Identifiers

PMID38384793
PMCPMC10879783

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.