ArticleScience advances2024
Phase-shifting optothermal microscopy enables live-cell mid-infrared hyperspectral imaging of large cell populations at high confluency.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Metasurface-enhanced biomedical spectroscopy.Nanophotonics (Berlin, Germany) · 2025Review
- Mid-Infrared Energy Deposition Spectroscopy.Physical review letters · 2025Article
- INSPIRE: Single-beam probed complementary vibrational bioimaging.Science advances · 2024Article
- A tutorial on optical photothermal infrared (O-PTIR) microscopy.APL photonics · 2024Article
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5 authors at 1 institution in 1 country.
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Abstract
Rapid live-cell hyperspectral imaging at large fields of view (FOVs) and high cell confluency remains challenging for conventional vibrational spectroscopy-based microscopy technologies. At the same time, imaging at high cell confluency and large FOVs is important for proper cell function and statistical significance of measurements, respectively. Here, we introduce phase-shifting mid-infrared optothermal microscopy (PSOM), which interprets molecular-vibrational information as the optical path difference induced by mid-infrared absorption and can take snapshot vibrational images over broad excitation areas at high live-cell confluency. By means of phase-shifting, PSOM suppresses noise to a quarter of current optothermal microscopy modalities to allow capturing live-cell vibrational images at FOVs up to 50 times larger than state of the art. PSOM also reduces illumination power flux density (PFD) down to four orders of magnitude lower than other conventional vibrational microscopy methods, such as coherent anti-Stokes Raman scattering (CARS), thus considerably decreasing the risk of cell photodamage.
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