Evidence map›Paper›PMID 38381339›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in Drosophila melanogaster.

Ines Lucia Patop, Michael Canori, Sebastian Kadener

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ines Lucia Patop *Brandeis University, Waltham, MA, USA.
Michael Canori *Brandeis University, Waltham, MA, USA.
Sebastian KadenerBrandeis University, Waltham, MA, USA. skadener@brandeis.edu.

Funding

Predoctoral Training in Cross-Disciplinary Molecular and Cellular BiologyT32GM139798 · NIGMS · BRANDEIS UNIVERSITY · PI Paul Garrity, Bruce L Goode · 2021 to 2026
$3.5M
NIGMS NIH HHS T32 GM139798
6 · The paper itself

Abstract

Studying circular RNAs' function in vivo has been challenging due to the lack of generic tools to manipulate their levels without affecting their linear counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdown with tissue-specific resolution in flies.

Indexed as

Drosophila melanogasterRNA, CircularAnimalsRNA, Small InterferingRNA, CircularRNA, Small InterferingCircRNACircular RNAsDrosophila melanogasterIn vivo knockdownOff-targetsshRNA

Identifiers

PMID38381339

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.