Evidence map›Paper›PMID 38379827›Full record

ArticleBio-protocol2024

A Protocol for Custom Biomineralization of Enzymes in Metal-Organic Frameworks (MOFs).

Zoe Armstrong, Drew Jordahl, Austin MacRae, Qiaobin Li, Mary Lenertz, Patrick Shen, Anastasiia Botserovska, Li Feng, Angel Ugrinov, Zhongyu Yang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 64% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Zoe ArmstrongDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Drew JordahlDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Austin MacRaeDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Qiaobin LiDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Mary LenertzDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Patrick ShenDavis Highschool, Fargo, ND, USA.
Anastasiia BotserovskaDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Li FengDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Angel UgrinovDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
Zhongyu YangDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, USA.
North Dakota State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme immobilization offers a number of advantages that improve biocatalysis; however, finding a proper way to immobilize enzymes is often a challenging task. Implanting enzymes in metal-organic frameworks (MOFs) via co-crystallization, also known as biomineralization, provides enhanced reusability and stability with minimal perturbation and substrate selectivity to the enzyme. Currently, there are limited metal-ligand combinations with a proper protocol guiding the experimental procedures. We have recently explored 10 combinations that allow custom immobilization of enzymes according to enzyme stability and activity in different metals/ligands. Here, as a follow-up of that work, we present a protocol for how to carry out custom immobilization of enzymes using the available combinations of metal ions and ligands. Detailed procedures to prepare metal ions, ligands, and enzymes for their co-crystallization, together with characterization and assessment, are discussed. Precautions for each experimental step and result analysis are highlighted as well. This protocol is important for enzyme immobilization in various research and industrial fields. Key features • A wide selection of metal ions and ligands allows for the immobilization of enzymes in metal-organic frameworks (MOFs) via co-crystallization. • Step-by-step enzyme immobilization procedure via co-crystallization of metal ions, organic linkers, and enzymes. • Practical considerations and experimental conditions to synthesize the enzyme@MOF biocomposites are discussed. • The demonstrated method can be generalized to immobilize other enzymes and find other metal ion/ligand combinations to form MOFs in water and host enzymes.

Indexed as

Aqueous phase coprecipitationBiomineralizationCo-crystallizationEnzyme immobilizationMOF

Identifiers

PMID38379827
PMCPMC10875352
OpenAlexW4390916458

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.