Evidence map›Paper›PMID 38379206›Full record

ArticleThe Laryngoscope2024

A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.

Mukund M Goyal, Sarek A Shen, Mohamed Lehar, Angela Martinez, Hakim Hiel, Canhui Wang, Yulin Liu, Chao Wang, Daniel Q Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Laryngoscope, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mukund M GoyalDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.ORCID 0000-0002-4836-5530
Sarek A ShenDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0001-6627-6139
Mohamed LeharDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Angela MartinezDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hakim HielDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Canhui WangDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Yulin LiuDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Chao WangDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Daniel Q SunDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Funding

RESEARCH TRAINING IN OTOLARYNGOLOGYT32DC000027 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI CAREY, JOHN PATRICK · 1989 to 2023
$5.5M
Rational Design of Magnetic Nanoparticles for Cochlear Drug DeliveryK08DC020780 · NIDCD · UNIVERSITY OF CINCINNATI · PI Daniel Q Sun · 2024 to 2026
$602k
NIDCD NIH HHS 5T32DC000027-33NIDCD NIH HHS K08 DC020780NIDCD NIH HHS K08DC020780NIDCD NIH HHS T32 DC000027
6 · The paper itself

Abstract

introductionThe round window membrane (RWM) presents a significant barrier to the local application of therapeutics to the inner ear. We demonstrate a benchtop preclinical RWM model and evaluate superparamagnetic iron oxide nanoparticles (SPIONs) as vehicles for magnetically assisted drug delivery.

methodsGuinea pig RWM explants were inset into a 3D-printed dual chamber benchtop device. Custom-synthesized 7-nm iron core nanoparticles were modified with different polyethylene glycol chains to yield two sizes of SPIONs (NP-PEG600 and NP-PEG3000) and applied to the benchtop model with and without a magnetic field. Histologic analysis of the RWM was performed using transmission electron microscopy (TEM) and confocal microscopy.

resultsOver a 4-h period, 19.5 ± 1.9% of NP-PEG3000 and 14.6 ± 1.9% of NP-PEG600 were transported across the guinea pig RWM. The overall transport increased by 1.45× to 28.4 ± 5.8% and 21.0 ± 2.0%, respectively, when a magnetic field was applied. Paraformaldehyde fixation of the RWM decreased transport significantly (NP-PEG3000: 7.6 ± 1.5%; NP-PEG600: 7.0 ± 1.6%). Confocal and electron microscopy analysis demonstrated nanoparticle localization throughout all cellular layers and layer-specific transport characteristics within RWM.

conclusionThe guinea pig RWM explant benchtop model allows for targeted and practical investigations of transmembrane transport in the development of nanoparticle drug delivery vehicles. The presence of a magnetic field increases SPION delivery by 45%-50% in a nanoparticle size- and cellular layer-dependent manner. LEVEL OF EVIDENCE: NA Laryngoscope, 134:3355-3362, 2024.

Indexed as

Drug Delivery SystemsRound Window, EarAnimalsEar, InnerGuinea PigsMagnetic Iron Oxide NanoparticlesMagnetite NanoparticlesMicroscopy, ConfocalMicroscopy, Electron, TransmissionPolyethylene GlycolsPrinting, Three-DimensionalMagnetite NanoparticlesPolyethylene Glycolsdrug deliveryex vivo modelhearing lossintratympanic deliverynanoparticle transportround window membranesuper‐paramagnetic iron oxide nanoparticles

Identifiers

PMID38379206
PMCPMC11875551

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.