ArticleParasites & vectors2024
Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance.
Article in Parasites & vectors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 20 citations in OpenAlex.
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- Molecular and morphologic characterization of Trichuris trichiura infecting free-roaming African vervets (Chlorocebus aethiops sabaeus) on the Caribbean Island of St. Kitts.PLoS neglected tropical diseases · 2026Article
- Multilocus Characterization of Human Ascaris in Pakistan Indicates Ongoing Introgression and Shared Global Haplotypes.Acta parasitologica · 2026Article
- Genome skimming of dog faecal samples reveals mitogenomes indistinguishable from those of red fox-derivedParasitology · 2026Article
- Molecular taxonomic characterisation ofFrontiers in parasitology · 2026Article
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- Molecular characterization of the prevalent soil-transmitted helminths in Narathiwat Province, southern Thailand.PloS one · 2026Article
- A Simple Yet Reliable 12S rRNA-Based Molecular Approach for Identifying Bat Species.Animals : an open access journal from MDPI · 2025Article
- Retrospective study of spirocercosis in coyotes (Canis latrans) in Costa Rica: epidemiology, pathological findings, and molecular characterization of Spirocerca lupi.Parasites & vectors · 2025Article
- Molecular characterization and reference mitogenome of the hookwormParasitology · 2025Article
- Based on Mitochondrial Genomes and Gene Order Rearrangements: Phylogenetic Relationships and Terrestrial Adaptability in Paguroidea (Crustacea: Decapoda).Ecology and evolution · 2025Article
- Article
- Molecular Characterization and Analysis of HumanHealth science reports · 2025Article
- Integrative taxonomy in helminth analysis: protocols and limitations in the twenty-first century.Parasites & vectors · 2025Review
- Unveiling Tick Diversity in Cattle in Cameroon: Emergence ofVeterinary sciences · 2025Article
- A newFrontiers in veterinary science · 2025Article
- Detection Methods for Pine Wilt Disease: A Comprehensive Review.Plants (Basel, Switzerland) · 2024Review
- Real-time qPCR coupled with high-resolution melting curve analysis for the detection of the internal transcribed spacer 1 of Angiostrongylus costaricensis.Parasitology research · 2024Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundNematodes of the Ascarididae, Ancylostomatidae and Onchocercidae families are parasites of human and veterinary importance causing infections with high prevalence worldwide. Molecular tools have significantly improved the diagnosis of these helminthiases, but the selection of genetic markers for PCR or metabarcoding purposes is often challenging because of the resolution these may show.
methodsNuclear 18S rRNA, internal transcribed spacers 1 (ITS-1) and 2 (ITS-2), mitochondrial gene cytochrome oxidase 1 (cox1) and mitochondrial rRNA genes 12S and 16S loci were studied for 30 species of the mentioned families. Accordingly, their phylogenetic interspecies resolution, pairwise nucleotide p-distances and sequence availability in GenBank were analyzed.
resultsThe 18S rRNA showed the least interspecies resolution since separate species of the Ascaris, Mansonella, Toxocara or Ancylostoma genus were intermixed in phylogenetic trees as opposed to the ITS-1, ITS-2, cox1, 12S and 16S loci. Moreover, pairwise nucleotide p-distances were significantly different in the 18S compared to the other loci, with an average of 99.1 ± 0.1%, 99.8 ± 0.1% and 98.8 ± 0.9% for the Ascarididae, Ancylostomatidae and Onchocercidae families, respectively. However, ITS-1 and ITS-2 average pairwise nucleotide p-distances in the three families ranged from 72.7% to 87.3%, and the cox1, 12S and 16S ranged from 86.4% to 90.4%. Additionally, 2491 cox1 sequences were retrieved from the 30 analyzed species in GenBank, whereas 212, 1082, 994, 428 and 143 sequences could be obtained from the 18S, ITS-1, ITS-2, 12S and 16S markers, respectively.
conclusionsThe use of the cox1 gene is recommended because of the high interspecies resolution and the large number of sequences available in databases. Importantly, confirmation of the identity of an unknown specimen should always be complemented with the careful morphological examination of worms and the analysis of other markers used for specific parasitic groups.
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