ArticlePLoS genetics2024
Dual mutations in the whitefly nicotinic acetylcholine receptor β1 subunit confer target-site resistance to multiple neonicotinoid insecticides.
Article in PLoS genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Two New Polyketides From Streptomyces sp. SA61: Isolation, Structure Elucidation, and Insecticidal Activity Against Bemisia tabaci.Chemistry & biodiversity · 2026Article
- Insecticide Resistance Dynamics and Gene Screening inInsects · 2026Article
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- Evolving resistance patterns in Tetranychus urticae and Bemisia tabaci in Greece.Pest management science · 2026Article
- Article
- Integrated evaluation of plant oils, entomopathogenic fungi and insecticides against rugose spiralling whitefly (Frontiers in insect science · 2026Article
- Standing genetic variation underlies divergence of nicotinic acetylcholine receptor subunits among cryptic species of thebioRxiv : the preprint server for biology · 2025Article
- Mechanisms and Genetic Drivers of Resistance of Insect Pests to Insecticides and Approaches to Its Control.Toxics · 2025Review
- The cys-loop ligand-gated ion channel gene superfamily of the Colorado potato beetle, Leptinotarsa decemlineata.BMC genomics · 2025Article
- Mutations of the complex I PSST target gene confers acaricide resistance and a fitness cost in Panonychus citri.BMC biology · 2025Article
- Field-evolved resistance to neonicotinoids in the mosquito, Anopheles gambiae, is associated with mutations of nicotinic acetylcholine receptor subunits combined with cytochrome P450-mediated detoxification.Pesticide biochemistry and physiology · 2024Article
- Field-evolved resistance to neonicotinoids in the mosquito,bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
28 authors at 8 institutions in 4 countries.
Funding
Abstract
Neonicotinoid insecticides, which target insect nicotinic acetylcholine receptors (nAChRs), have been widely and intensively used to control the whitefly, Bemisia tabaci, a highly damaging, globally distributed, crop pest. This has inevitably led to the emergence of populations with resistance to neonicotinoids. However, to date, there have been no reports of target-site resistance involving mutation of B. tabaci nAChR genes. Here we characterize the nAChR subunit gene family of B. tabaci and identify dual mutations (A58T&R79E) in one of these genes (BTβ1) that confer resistance to multiple neonicotinoids. Transgenic D. melanogaster, where the native nAChR Dβ1 was replaced with BTβ1A58T&R79E, were significantly more resistant to neonicotinoids than flies where Dβ1 were replaced with the wildtype BTβ1 sequence, demonstrating the causal role of the mutations in resistance. The two mutations identified in this study replace two amino acids that are highly conserved in >200 insect species. Three-dimensional modelling suggests a molecular mechanism for this resistance, whereby A58T forms a hydrogen bond with the R79E side chain, which positions its negatively-charged carboxylate group to electrostatically repulse a neonicotinoid at the orthosteric site. Together these findings describe the first case of target-site resistance to neonicotinoids in B. tabaci and provide insight into the molecular determinants of neonicotinoid binding and selectivity.
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