Evidence map›Paper›PMID 38376381›Full record

ArticleMicrobial genomics2024

Closing the genome of unculturable cable bacteria using a combined metagenomic assembly of long and short sequencing reads.

Anwar Hiralal, Jeanine S Geelhoed, Silvia Hidalgo-Martinez, Bent Smets, Jesper R van Dijk, Filip J R Meysman

Abstract read
In one paragraph

Article in Microbial genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Metagenome-assembled genome sequence ofMicrobiology resource announcements · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anwar HiralalGeobiology Research Group, University of Antwerp, Antwerp, Belgium.
Jeanine S GeelhoedGeobiology Research Group, University of Antwerp, Antwerp, Belgium.
Silvia Hidalgo-MartinezGeobiology Research Group, University of Antwerp, Antwerp, Belgium.
Bent SmetsGeobiology Research Group, University of Antwerp, Antwerp, Belgium.
Jesper R van DijkGeobiology Research Group, University of Antwerp, Antwerp, Belgium.
Filip J R MeysmanGeobiology Research Group, University of Antwerp, Antwerp, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many environmentally relevant micro-organisms cannot be cultured, and even with the latest metagenomic approaches, achieving complete genomes for specific target organisms of interest remains a challenge. Cable bacteria provide a prominent example of a microbial ecosystem engineer that is currently unculturable. They occur in low abundance in natural sediments, but due to their capability for long-distance electron transport, they exert a disproportionately large impact on the biogeochemistry of their environment. Current available genomes of marine cable bacteria are highly fragmented and incomplete, hampering the elucidation of their unique electrogenic physiology. Here, we present a metagenomic pipeline that combines Nanopore long-read and Illumina short-read shotgun sequencing. Starting from a clonal enrichment of a cable bacterium, we recovered a circular metagenome-assembled genome (5.09 Mbp in size), which represents a novel cable bacterium species with the proposed name

Indexed as

DeltaproteobacteriaMetagenomeBacteriaEcosystemHigh-Throughput Nucleotide Sequencingcable bacteriaCandidatus Electrothrix scaldisclonal enrichmentgenome closurehybrid sequencingmetagenomics

Identifiers

PMID38376381
PMCPMC10926707

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.