Evidence map›Paper›PMID 38375546›Full record

ArticleMicrobiologyOpen2024

Effect of the polysaccharide capsule and its heptose on the resistance of Campylobacter jejuni to innate immune defenses.

Matthew Myles, Heba Barnawi, Mahmoud Mahmoudpour, Sargon Shlimon, Adrienne Chang, Daniel Zimmermann, Chiwon Choi, Najwa Zebian, Carole Creuzenet

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

  1. A Review of Essential Oils with Anti-Foods (Basel, Switzerland) · 2026
    Review
  2. Article
  3. Article
  4. Small worms big discoveries:Frontiers in cellular and infection microbiology · 2025
    Review
  5. Consortium ofAntibiotics (Basel, Switzerland) · 2024
    Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Matthew MylesMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Heba BarnawiMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Mahmoud MahmoudpourMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.ORCID 0000-0002-1198-0126
Sargon ShlimonMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Adrienne ChangMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Daniel ZimmermannMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Chiwon ChoiMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Najwa ZebianMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
Carole CreuzenetMicrobiology and Immunology, The University of Western Ontario, London, Ontario, Canada.ORCID 0000-0002-7435-5862
Western University · CA

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN 04765-2016Natural Sciences and Engineering Research Council of Canada RGPIN 05717-2015Natural Sciences and Engineering Research Council of Canada RGPIN 240762-2010
6 · The paper itself

Abstract

Campylobacter jejuni is a commensal in many animals but causes diarrhea in humans. Its polysaccharide capsule contributes to host colonization and virulence in a strain- and model-specific manner. We investigated if the capsule and its heptose are important for interactions of strain NCTC 11168 with various hosts and their innate immune defenses. We determined that they support bacterial survival in Drosophila melanogaster and enhance virulence in Galleria mellonella. We showed that the capsule had limited antiphagocytic activity in human and chicken macrophages, decreased adherence to chicken macrophages, and decreased intracellular survival in both macrophages. In contrast, the heptose increased uptake by chicken macrophages and supported adherence to human macrophages and survival within them. While the capsule triggered nitric oxide production in chicken macrophages, the heptose mitigated this and protected against nitrosative assault. Finally, the C. jejuni strain NCTC 11168 elicited strong cytokine production in both macrophages but quenched ROS production independently from capsule and heptose, and while the capsule and heptose did not protect against oxidative assault, they favored growth in biofilms under oxidative stress. This study shows that the wild-type capsule with its heptose is optimized to resist innate defenses in strain NCTC 11168 often via antagonistic effects of the capsule and its heptose.

Indexed as

Campylobacter InfectionsCampylobacter jejuniAnimalsChickensDrosophila melanogasterHeptosesHumansImmunity, InnatePolysaccharidesHeptosesPolysaccharidesCampylobacter jejunicapsuleheptoseinnate immune defensesmacrophagesredox stress

Identifiers

PMID38375546
PMCPMC10877309
OpenAlexW4391966823

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.