ArticleThe Plant cell2024
Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Development and regulatory approval of Kal91.3: combining advanced conventional breeding with genetic engineering to create a cold sweetening‑resistant chip‑processing potato.GM crops & food · 2026Article
- Positional grammar of transcription factor binding partitions developmental and stress-response regulation in plants.Nature communications · 2026Article
- Epigenetic orchestration of sugar signaling in plant development and stress adaptation.Horticulture research · 2026Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- A GLUTAMATE CYSTEINE LIGASE Gene StGSH1 Regulated by StERF10 Enhanced Glutathione Accumulation and Adaptation to Low Phosphorus Stress in Potato.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Research progress on cold tolerance key genes and mechanisms in four major northern China crops.Frontiers in plant science · 2026Review
- CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025Review
- Molecular Mechanisms Underlying Defense Responses of Potato (Plants (Basel, Switzerland) · 2025Review
- Enhancers in Plant Development, Adaptation and Evolution.Plant & cell physiology · 2025Review
- CRISPR/Cas9-Mediated Development of Potato Varieties with Long-Term Cold Storage and Bruising Resistance.Biology · 2025Article
- Jan and mini-Jan, a model system for potato functional genomics.Plant biotechnology journal · 2025Article
- Article
- Roles and Regulations of Acid Invertases in Plants: Current Knowledge and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- The Arabidopsis thaliana core splicing factor PORCUPINE/SmE1 requires intron-mediated expression.PloS one · 2025Article
- Deciphering the regulatory mechanisms of potato cold-induced sweetening via integrated time-course transcriptome and metabolome analysis.Frontiers in plant science · 2025Article
- Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato.Frontiers in genome editing · 2025Article
- Jan and mini-Jan, a model system for potato functional genomics.bioRxiv : the preprint server for biology · 2024Article
- Review
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to minimize sprouting and losses due to disease. However, cold temperatures strongly induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulation. This process, referred to as "cold-induced sweetening," is a major postharvest problem for the potato industry. We discovered that the cold-induced expression of VInv is controlled by a 200 bp enhancer, VInvIn2En, located in its second intron. We identified several DNA motifs in VInvIn2En that bind transcription factors involved in the plant cold stress response. Mutation of these DNA motifs abolished VInvIn2En function as a transcriptional enhancer. We developed VInvIn2En deletion lines in both diploid and tetraploid potato using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated gene editing. VInv transcription in cold-stored tubers was significantly reduced in the deletion lines. Interestingly, the VInvIn2En sequence is highly conserved among distantly related Solanum species, including tomato (Solanum lycopersicum) and other non-tuber-bearing species. We conclude that the VInv gene and the VInvIn2En enhancer have adopted distinct roles in the cold stress response in tubers of tuber-bearing Solanum species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.