ArticleScientific reports2024
CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Present and Future of Mosquito-Borne Disease Control in Europe with a Specific Focus on the Mediterranean.Insects · 2026Review
- Emerging trends in genome editing of wild animals.Transgenic research · 2026Review
- Article
- CRISPR Technology: Transforming the Future of Medicine and Diagnostics.Biochemistry · 2025Review
- Article
- Engineering gene drive docking sites in a haplolethal locus in Anopheles gambiae.Scientific reports · 2025Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Defining the genetics of the widely used G3 strain of the mosquito, Anopheles gambiae.Scientific reports · 2025Article
- ExploitingPathogens (Basel, Switzerland) · 2025Review
- Precision-guided tools for malaria control.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives.Nature communications · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
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9 authors at 3 institutions in 1 country.
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Abstract
Rapid spread of insecticide resistance among anopheline mosquitoes threatens malaria elimination efforts, necessitating development of alternative vector control technologies. Sterile insect technique (SIT) has been successfully implemented in multiple insect pests to suppress field populations by the release of large numbers of sterile males, yet it has proven difficult to adapt to Anopheles vectors. Here we outline adaptation of a CRISPR-based genetic sterilization system to selectively ablate male sperm cells in the malaria mosquito Anopheles gambiae. We achieve robust mosaic biallelic mutagenesis of zero population growth (zpg, a gene essential for differentiation of germ cells) in F1 individuals after intercrossing a germline-expressing Cas9 transgenic line to a line expressing zpg-targeting gRNAs. Approximately 95% of mutagenized males display complete genetic sterilization, and cause similarly high levels of infertility in their female mates. Using a fluorescence reporter that allows detection of the germline leads to a 100% accurate selection of spermless males, improving the system. These males cause a striking reduction in mosquito population size when released at field-like frequencies in competition cages against wild type males. These findings demonstrate that such a genetic system could be adopted for SIT against important malaria vectors.
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