Evidence map›Paper›PMID 38374224›Full record

ArticleNature biomedical engineering2024

Extracellular vesicles incorporating retrovirus-like capsids for the enhanced packaging and systemic delivery of mRNA into neurons.

Wenchao Gu, Sijin Luozhong, Simian Cai, Ketaki Londhe, Nadine Elkasri, Robert Hawkins, Zhefan Yuan, Kai Su-Greene, Yujie Yin, Margaret Cruz and 14 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Nature biomedical engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed.

  1. Review
  2. Targeting mNature communications · 2026
    Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
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  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

24 authors.

Wenchao GuMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Sijin LuozhongMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0001-8697-9480
Simian CaiDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0001-9686-0635
Ketaki LondheMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Nadine ElkasriMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0002-4619-2429
Robert HawkinsMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Zhefan YuanMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Kai Su-GreeneDepartment of Molecular Medicine, Cornell University, Ithaca, NY, USA.
Yujie YinRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
Margaret CruzMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Yu-Wei ChangDepartment of Biomedical Sciences, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0003-3333-5424
Patrick McMullenMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Chunyan WuDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0002-6294-4512
Changwoo SeoDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.
Akash GuruDepartment of Neurobiology and Behavior, Cornell University, Ithaca, NY, USA.
Wenting GaoDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, USA.
Tara SarmientoMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Chris SchafferMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0002-7800-9596
Nozomi NishimuraMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0003-4342-9416
Richard CerioneDepartment of Molecular Medicine, Cornell University, Ithaca, NY, USA.
Qiuming YuRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
Melissa WardenDepartment of Biomedical Sciences, Cornell University, Ithaca, NY, USA.
Robert LangerDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. rlanger@mit.edu.ORCID http://orcid.org/0000-0003-4255-0492
Shaoyi JiangMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. sj19@cornell.edu.ORCID http://orcid.org/0000-0001-9863-6899

Funding

Cornell University Cornell NEXT nano initiativeCornell University Robert Langer '70 Family and Friends ProfessorshipCornell University Sprout Award
6 · The paper itself

Abstract

The blood-brain barrier (BBB) restricts the systemic delivery of messenger RNAs (mRNAs) into diseased neurons. Although leucocyte-derived extracellular vesicles (EVs) can cross the BBB at inflammatory sites, it is difficult to efficiently load long mRNAs into the EVs and to enhance their neuronal uptake. Here we show that the packaging of mRNA into leucocyte-derived EVs and the endocytosis of the EVs by neurons can be enhanced by engineering leucocytes to produce EVs that incorporate retrovirus-like mRNA-packaging capsids. We transfected immortalized and primary bone-marrow-derived leucocytes with DNA or RNA encoding the capsid-forming activity-regulated cytoskeleton-associated (Arc) protein as well as capsid-stabilizing Arc 5'-untranslated-region RNA elements. These engineered EVs inherit endothelial adhesion molecules from donor leukocytes, recruit endogenous enveloping proteins to their surface, cross the BBB, and enter the neurons in neuro-inflammatory sites. Produced from self-derived donor leukocytes, the EVs are immunologically inert, and enhanced the neuronal uptake of the packaged mRNA in a mouse model of low-grade chronic neuro-inflammation.

Indexed as

Blood-Brain BarrierExtracellular VesiclesNeuronsRNA, MessengerAnimalsCapsidHumansLeukocytesMiceMice, Inbred C57BLRetroviridaeRNA, Messenger

Identifiers

PMID38374224

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.