Evidence map›Paper›PMID 38374072›Full record

ArticleJournal of nanobiotechnology2024

M2 macrophage-derived exosomal miR-26b-5p regulates macrophage polarization and chondrocyte hypertrophy by targeting TLR3 and COL10A1 to alleviate osteoarthritis.

Yufan Qian, Genglei Chu, Lei Zhang, Zhikai Wu, Qiuyuan Wang, Jiong Jiong Guo, Feng Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 1 pooled it
36.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.

  1. Pooled it
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  6. [Advances in immunomodulatory strategies for meniscal regeneration].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2026
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yufan Qian *Department of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China.
Genglei Chu *Department of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China.
Lei Zhang *Department of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China.
Zhikai WuDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China.
Qiuyuan WangDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China.
Jiong Jiong GuoDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China. drjjguo@163.com.
Feng ZhouDepartment of Orthopaedics, The First Affiliated Hospital of Soochow University, No. 899 Ping Hai Road, Suzhou, Jiangsu, China. sdfyyzhoufeng@163.com.
Soochow University · CNFirst Affiliated Hospital of Soochow University · CN

Funding

Gusu Health Talent Project of Suzhou GSWS2022004the Clinical Medicine Peak Program of Suzhou Medical College of Suzhou University ML12201323the National Natural Science Foundation of China 82102606the Natural Science Foundation of Jiangsu Province BK20210093
6 · The paper itself

Abstract

Osteoarthritis (OA) is one of the most prevalent chronic musculoskeletal diseases among the elderly population. In this study, macrophage-derived exosomes were isolated and identified. Exosomes were subjected to microRNA (miRNA) sequencing and bioinformatic analysis, and differentially expressed miRNAs were verified. miR-26b-5p target genes were confirmed through target-site mutation combined with a dual-luciferase reporter assay. The effects of miR-26b-5p on macrophage polarization and chondrocyte hypertrophy were assessed in vitro. miR-26b-5p agomir was applied to mice with OA induced by anterior cruciate ligament transection (ACLT). The therapeutic effects of miR-26b-5p were evaluated via pain behavior experiments and histological observations. In vitro, miR-26b-5p repolarized M1 macrophages to an anti-inflammatory M2 type by targeting the TLR3 signaling pathway. miR-26b-5p could target COL10A1, further inhibiting chondrocyte hypertrophy induced by M1 macrophage-conditioned medium (M1-CM). In vivo, miR-26b-5p agomir ameliorated gait abnormalities and mechanical allodynia in OA mice. miR-26b-5p treatment attenuated synovitis and cartilage degeneration, thereby delaying OA progression. In conclusion, M2 macrophage-derived exosomal miR-26b-5p could protect articular cartilage and ameliorate gait abnormalities in OA mice by targeting TLR3 and COL10A1. miR-26b-5p further affected macrophage polarization and chondrocyte hypertrophy. Thus, this exosomal miR-26b-5p-based strategy might be a potential method for OA treatment.

Indexed as

MicroRNAsOsteoarthritisAgedAnimalsChondrocytesCollagen Type XExosomesHumansHypertrophyMacrophagesMiceToll-Like Receptor 3Col10a1 protein, mouseCollagen Type XMicroRNAsTLR3 protein, humanTLR3 protein, mouseToll-Like Receptor 3Chondrocyte hypertrophyExosomal miR-26b-5pMacrophage polarizationOsteoarthritisPain behavior

Identifiers

PMID38374072
PMCPMC10877765
OpenAlexW4391946263

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.