ArticleLangmuir : the ACS journal of surfaces and colloids2024
Bilayer Charge Asymmetry and Oil Residues Destabilize Membranes upon Poration.
Article in Langmuir : the ACS journal of surfaces and colloids, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Electric-field manipulation of giant vesicles: new frontiers in membrane electromechanics.European biophysics journal : EBJ · 2026Review
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Analysis of Inner Content and Phospholipid Membrane Fusion during Two Step Freezing Induced Liposome Fusion.ACS omega · 2026Article
- Elucidating the mechanical properties of asymmetric membranes by direct derivation of their energetics.Faraday discussions · 2025Article
- Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates.Nature communications · 2025Article
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
- Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmembrane asymmetry is ubiquitous in cells, particularly with respect to lipids, where charged lipids are mainly restricted to one monolayer. We investigate the influence of anionic lipid asymmetry on the stability of giant unilamellar vesicles (GUVs), minimal plasma membrane models. To quantify asymmetry, we apply the fluorescence quenching assay, which is often difficult to reproduce, and caution in handling the quencher is generally underestimated. We first optimize this assay and then apply it to GUVs prepared with the inverted emulsion transfer protocol by using increasing fractions of anionic lipids restricted to one leaflet. This protocol is found to produce highly asymmetric bilayers but with ∼20% interleaflet mixing. To probe the stability of asymmetric versus symmetric membranes, we expose the GUVs to porating electric pulses and monitor the fraction of destabilized vesicles. The pulses open macropores, and the GUVs either completely recover or exhibit leakage or bursting/collapse. Residual oil destabilizes porated membranes, and destabilization is even more pronounced in asymmetrically charged membranes. This is corroborated by the measured pore edge tension, which is also found to decrease with increasing charge asymmetry. Using GUVs with imposed transmembrane pH asymmetry, we confirm that poration-triggered destabilization does not depend on the approach used to generate membrane asymmetry.
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