ArticleThe Journal of cell biology1979
Changing patterns of plasma membrane-associated filaments during the initial phases of polymorphonuclear leukocyte adherence.
Article in The Journal of cell biology, 1979. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Cell membrane alignment along adhesive surfaces: contribution of active and passive cell processes.Biophysical journal · 2003Article
- Microtubule asymmetry during neutrophil polarization and migration.Molecular biology of the cell · 2002Article
- A massage for the journey: keeping leukocytes soft and silent.Proceedings of the National Academy of Sciences of the United States of America · 1997Review
- Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes.Molecular biology of the cell · 1995Article
- Transient increases in cytosolic free calcium appear to be required for the migration of adherent human neutrophils.The Journal of cell biology · 1990Article
- Polymorphonuclear leukocyte adherence induces actin polymerization by a transduction pathway which differs from that used by chemoattractants.The Journal of cell biology · 1989Article
- Cytosolic free calcium increases before and oscillates during frustrated phagocytosis in macrophages.The Journal of cell biology · 1987Article
- Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.The Journal of cell biology · 1985Article
- Organization of the cytoskeleton in resting, discoid platelets: preservation of actin filaments by a modified fixation that prevents osmium damage.The Journal of cell biology · 1985Article
- Relationship of actin polymerization and depolymerization to light scattering in human neutrophils: dependence on receptor occupancy and intracellular Ca++.The Journal of cell biology · 1985Article
- Distribution of epinemin in colloidal gold-labelled, quick-frozen, deep-etched cytoskeletons.The Journal of cell biology · 1984Article
- Optimal techniques for the immunocytochemical demonstration of beta-thromboglobulin, platelet factor 4, and fibrinogen in the alpha granules of unstimulated platelets.The Histochemical journal · 1984Article
- Epinemin: a new protein associated with vimentin filaments in non-neural cells.The Journal of cell biology · 1983Article
- High-resolution three-dimensional views of membrane-associated clathrin and cytoskeleton in critical-point-dried macrophages.The Journal of cell biology · 1983Article
- Visualization of antigens attached to cytoskeletal framework in animal cells: colocalization of simian virus 40 Vp1 polypeptide and actin in TC7 cells.Proceedings of the National Academy of Sciences of the United States of America · 1983Article
- Three-dimensional structure of actin filaments and of an actin gel made with actin-binding protein.The Journal of cell biology · 1983Article
- The role of cytoskeletal and cytocontractile elements in pathologic processes.The American journal of pathology · 1983Review
- Distribution of actin in spreading macrophages: a comparative study on living and fixed cells.The Journal of cell biology · 1983Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By utilizing a combination of several ultrastructural techniques, we have been able to demonstrate differences in filament organization on the adherent plasma membranes of spreading and mobile PMN as well as within the extending lamellipodia. To follow the subplasmalemmal filaments of this small amoeboid cell during these kinetic events, we sheared off the upper portions of cells onto glass and carbon surfaces for 30 s--5 min. The exposed adherent membranes were immediately fixed and processed for high-resolution SEM or TEM. Whole cells were also examined by phase contrast microscopy, SEM, and oriented thin sections. Observed by SEM, the inner surface of nonadherent PMN membranes is free of filaments, but within 30 s of attachment to the substrate a three-dimensional, interlocking network of globular projections and radiating microfilaments--i.e., a subplasmalemmal filament complex--is consistently demonstrable (with or without postfixation in OsO4). Seen by TEM, extending lamellipodia contain a felt of filamentous and finely granular material, distinct from the golbule/filament complex of the adjacent adherent membrane. In the spread cell, this golbule-filament complex covers the entire lower membrane and increases in filament-density over the next 2--3 min. By 3--5 min after plating, as the PMN rounds up before the initiation of amoeboid movements, another pattern emerges--circumferential bands of anastomosing filament bundles in which thick, short filaments resembling myosin are found. This work provides structural evidence on the organization of polymerized contractile elements associated with the plasma membrane during cellular adherence.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.