ArticleFood science and biotechnology2024
Quantitative real-time PCR analysis of four genetically modified soybean events using plasmid and genomic DNA calibrators.
Article in Food science and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Screening method for detection of genetically modified soybean and maize events using multiplex PCR combined with capillary electrophoresis.GM crops & food · 2026Article
- A standard-curve-free ΔΔCt method for rapid GMO compliance screening relative to labeling thresholds.Frontiers in plant science · 2026Article
- Development and validation of an event-specific detection method for WYN029GmA soybean based on TaqMan qPCR.Frontiers in plant science · 2026Article
- Development of PMAxx-droplet digital PCR method for the absolute quantification of viableFrontiers in microbiology · 2026Article
- Specific detection of genetically modified potatoes containingGM crops & food · 2025Article
- In vitro evaluation of CeODaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2025Article
- Development and verification of new reference plasmid (pUC_GM-SB) for the DNA-based detection of genetically modified sugar beet H7-1.Food science and biotechnology · 2024Article
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3 authors.
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Abstract
A calibration curve is required for reliable and accurate quantitative real-time PCR analysis of genetically modified (GM) organisms, necessitating the use of reference materials as calibrators. In this study, two types of DNA calibrators-plasmid DNA (pDNA) and genomic DNA (gDNA)-were used to quantify four GM soybean events (SYHT0H2, MON87751, DAS-44406-6, and DAS-81419-2). The PCR efficiency and linearity for the calibrators adhered to the CODEX guidelines. The conversion factor (Cf) was calculated as the ratio of copies of GM events to those of endogenous genes using the pDNA calibration curve. To assess the accuracy and repeatability of these assays, quantification at GM levels of 3% and 1% was performed. Based on our results, we believe that the pDNA calibrator assessed in this study can be used as a reference material for GMO quantitative analysis and can replace gDNA, especially considering the ease of management and advantages of mass production. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-023-01392-0.
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