Evidence map›Paper›PMID 38370785›Full record

ArticlebioRxiv : the preprint server for biology2024

Protein-protein interactions with G3BPs drive stress granule condensation and gene expression changes under cellular stress.

José M Liboy-Lugo, Carla A Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E Park, Albert Xu, Ziad Jowhar, Angela L Gao, José A Carmona-Negrón, Torsten Wittmann, Natalia Jura and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 2 countries.

José M Liboy-LugoDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-9997-2285
Carla A EspinozaTetrad Graduate Program, University of California, San Francisco, San Francisco, California, USA.
Jessica Sheu-GruttadauriaDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-6806-4532
Jesslyn E ParkDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.
Albert XuDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.
Ziad JowharDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-3536-079X
Angela L GaoTetrad Graduate Program, University of California, San Francisco, San Francisco, California, USA.
José A Carmona-NegrónCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Torsten WittmannDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0001-9134-691X
Natalia JuraCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0001-5129-641X
Stephen N FloorDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-9965-9694
University of California, San Francisco · US

Funding

Medical Scientist Training Program (T32 NRSA Training Grant)T32GM141323 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Aimee Kao · 2021 to 2026
$10.5M
Wide-field super-resolution spinning disk confocal microscopeS10OD028611 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2020 to 2020
$600k
Illumina NovaSeq 6000 Sequencing SystemS10OD028511 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHOW, ERIC D · 2020 to 2020
$583k
The molecular grammar of human RNA biologyR35GM149255 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FLOOR, STEPHEN NICHOLAS · 2023 to 2024
$498k
The role of G3BP granules in mRNA translation regulation and cell adaptation to exogenous stressF31GM143845 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIBOY, JOSE · 2021 to 2023
$125k
NIGMS NIH HHS F31 GM143845NIGMS NIH HHS R35 GM149255NIGMS NIH HHS T32 GM141323NIH HHS S10 OD028511NIH HHS S10 OD028611
6 · The paper itself

Abstract

Stress granules (SGs) are macromolecular assemblies that form under cellular stress. Formation of these condensates is driven by the condensation of RNA and RNA-binding proteins such as G3BPs. G3BPs condense into SGs following stress-induced translational arrest. Three G3BP paralogs (G3BP1, G3BP2A, and G3BP2B) have been identified in vertebrates. However, the contribution of different G3BP paralogs to stress granule formation and stress-induced gene expression changes is incompletely understood. Here, we identified key residues for G3BP condensation such as V11. This conserved amino acid is required for formation of the G3BP-Caprin-1 complex, hence promoting SG assembly. Total RNA sequencing and ribosome profiling revealed that disruption of G3BP condensation corresponds to changes in mRNA levels and ribosome engagement during the integrated stress response (ISR). Moreover, we found that G3BP2B preferentially condenses and promotes changes in mRNA expression under endoplasmic reticulum (ER) stress. Together, this work suggests that stress granule assembly promotes changes in gene expression under cellular stress, which is differentially regulated by G3BP paralogs.

Indexed as

G3BPsintegrated stress responseribosome profilingStress granules

Identifiers

PMID38370785
PMCPMC10871250
OpenAlexW4391615511

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.