Evidence map›Paper›PMID 38370693›Full record

ArticlebioRxiv : the preprint server for biology2024

Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM grid plunge freezing.

Kaitlyn M Abe, Ci Ji Lim

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kaitlyn M AbeDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.ORCID 0000-0001-6055-5604
Ci Ji LimDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.ORCID 0000-0003-3327-1926
University of Wisconsin–Madison · US

Funding

Unraveling the Telomere Black Box: A New Single-Molecule Approach to Define the Telomere Chromatin Landscape and its Functional MechanismsDP2GM150023 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI LIM, CI JI · 2022 to 2025
$2.3M
Predoctoral Training in Molecular BiophysicsT32GM130550 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SENES, ALESSANDRO · 2019 to 2023
$1.5M
Structural understanding of human shelterin complex assembly at telomere and its regulation mechanism of telomerase activityR00GM131023 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI LIM, CI JI · 2020 to 2022
$730k
NIGMS NIH HHS DP2 GM150023NIGMS NIH HHS R00 GM131023NIGMS NIH HHS T32 GM130550
6 · The paper itself

Abstract

Sample loss due to air-water interface (AWI) interactions is a significant challenge during cryo-electron microscopy (cryo-EM) sample grid plunge freezing. We report that small Late Embryogenesis Abundant (LEA) proteins, which naturally bind to AWI, can protect samples from AWI damage during plunge freezing. This protection is demonstrated with two LEA proteins from nematodes and tardigrades, which rescued the cryo-EM structural determination outcome of two fragile multisubunit protein complexes.

Identifiers

PMID38370693
PMCPMC10871254
OpenAlexW4391792260

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.