Evidence map›Paper›PMID 38370615›Full record

ArticlebioRxiv : the preprint server for biology2024

Sparse CBX2 nucleates many Polycomb proteins to promote facultative heterochromatinization of Polycomb target genes.

Steven Ingersoll, Abby Trouth, Xinlong Luo, Axel Espinoza, Joey Wen, Joseph Tucker, Kalkidan Astatike, Christopher J Phiel, Tatiana G Kutateladze, Tao P Wu and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Steven IngersollDepartment of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA.
Abby TrouthDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA.
Xinlong LuoDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Axel EspinozaDepartment of Integrative Biology, University of Colorado Denver, CO 80217-3364, USA.
Joey WenDepartment of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA.
Joseph TuckerDepartment of Integrative Biology, University of Colorado Denver, CO 80217-3364, USA.
Kalkidan AstatikeDepartment of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA.
Christopher J PhielDepartment of Integrative Biology, University of Colorado Denver, CO 80217-3364, USA.
Tatiana G KutateladzeDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Tao P WuDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Srinivas RamachandranDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA.
Xiaojun RenDepartment of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA.
University of Colorado Denver · USUniversity of Denver · USBaylor College of Medicine · US

Funding

Maintenance of cellular memory through replicationR35GM133434 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI RAMACHANDRAN, SRINIVAS · 2019 to 2023
$2.0M
Spatial Organization of Epigenetic Complexes and ChromatinR01GM135286 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI PHIEL, CHRISTOPHER J · 2019 to 2022
$1.3M
NIGMS NIH HHS R01 GM135286NIGMS NIH HHS R35 GM133434
6 · The paper itself

Abstract

Facultative heterochromatinization of genomic regulators by Polycomb repressive complex (PRC) 1 and 2 is essential in development and differentiation; however, the underlying molecular mechanisms remain obscure. Using genetic engineering, molecular approaches, and live-cell single-molecule imaging, we quantify the number of proteins within condensates formed through liquid-liquid phase separation (LLPS) and find that in mouse embryonic stem cells (mESCs), approximately 3 CBX2 proteins nucleate many PRC1 and PRC2 subunits to form one non-stoichiometric condensate. We demonstrate that sparse CBX2 prevents Polycomb proteins from migrating to constitutive heterochromatin, demarcates the spatial boundaries of facultative heterochromatin, controls the deposition of H3K27me3, regulates transcription, and impacts cellular differentiation. Furthermore, we show that LLPS of CBX2 is required for the demarcation and deposition of H3K27me3 and is essential for cellular differentiation. Our findings uncover new functional roles of LLPS in the formation of facultative heterochromatin and unravel a new mechanism by which low-abundant proteins nucleate many other proteins to form compartments that enable them to execute their functions.

Indexed as

CBX2chromatinconstitutive heterochromatinfacultative heterochromatinH3K27me3liquid-liquid phase separationlive-cell single-molecule trackingnucleationPolycombPRC1PRC2single-molecule imaging

Identifiers

PMID38370615
PMCPMC10871256
OpenAlexW4391572697

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.