Evidence map›Paper›PMID 38369725›Full record

ArticleCombinatorial chemistry & high throughput screening2024

(S)-10-Hydroxycamptothecin Inhibits EMT-evoked Osteosarcoma Cell Growth and Metastasis by Activating the HIPPO Signaling Pathway.

Xiaoping Ma, Junli Chang, Xingyuan Sun, Chujie Zhou, Peng Zhao, Yanping Yang

Abstract read
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In one paragraph

Article in Combinatorial chemistry & high throughput screening, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Xiaoping MaLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Junli ChangLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Xingyuan SunLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Chujie ZhouLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Peng ZhaoLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Yanping YangLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
Ministry of Education of the People's Republic of China · CNLonghua Hospital Shanghai University of Traditional Chinese Medicine · CNShanghai University of Traditional Chinese Medicine · CN

Funding

National Natural Science Foundation of China (NSFC) (81973877 and 82174408)Shanghai University of Traditional Chinese Medicine (2021LK047).
6 · The paper itself

Abstract

backgroundOsteosarcoma is the most common primary bone cancer in children and adolescents with high metastatic ability.

aimThis study aimed to explore the inhibitory effects of (S)-10-hydroxycamptothecin (HCPT) on osteosarcoma cell growth and metastasis as well as the underlying mechanism.

methodsThe osteosarcoma cells of 143B and U-2 OS (U-2), treated with HCPT (20, 100, or 300 nM), underwent detections, such as CCK-8, flow cytometry, Transwell, wound healing, and immunoblotting. EMT-related key proteins, like N-cadherin, Snail, and Vimentin, were found to be down-regulated, while E-cadherin was up-regulated dose-dependently in HCPT-exposed 143B and U-2 cells. Additionally, incubation of 143B and U-2 cells with HCPT for 3 hours dosedependently reduced the expression ratios of p-LATS1/LATS1, p-MST1/MST1, p-YAP/YAP, and p-TAZ/TAZ.

resultsTaken together, our study has demonstrated HCPT to inhibit osteosarcoma growth and metastasis potentially by activating the HIPPO signaling pathway and reversing EMT.

conclusionHCPT might be a candidate agent for the prevention and treatment of osteosarcoma.

Indexed as

CamptothecinCell ProliferationEpithelial-Mesenchymal TransitionHippo Signaling PathwayOsteosarcomaSignal TransductionBone NeoplasmsCell Line, TumorDose-Response Relationship, DrugDrug Screening Assays, AntitumorHumansProtein Serine-Threonine Kinases10-hydroxycamptothecinCamptothecinProtein Serine-Threonine KinasesEMTHIPPOlarge tumor suppressor.metastasisOsteosarcoma(S)-10-hydroxycamptothecin

Identifiers

PMID38369725
OpenAlexW4391324246

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.