Evidence map›Paper›PMID 38368393›Full record

ArticleJournal of nanobiotechnology2024

Exosome lncRNA IFNG-AS1 derived from mesenchymal stem cells of human adipose ameliorates neurogenesis and ASD-like behavior in BTBR mice.

Yu Fu, Yuan-Lin Zhang, Rong-Qi Liu, Meng-Meng Xu, Jun-Ling Xie, Xing-Liao Zhang, Guang-Ming Xie, Yao-Ting Han, Xin-Min Zhang, Wan-Ting Zhang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 2 pooled it
6.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yu Fu *Research Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Yuan-Lin Zhang *Research Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Rong-Qi LiuResearch Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200010, China.
Meng-Meng XuResearch Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Jun-Ling XieResearch Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Xing-Liao ZhangResearch Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Guang-Ming XieResearch Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China.
Yao-Ting HanResearch Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200010, China.
Xin-Min ZhangResearch Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200010, China.
Wan-Ting ZhangResearch Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200010, China.
Jing ZhangResearch Center for Translational Medicine at East Hospital, School of Life Science and Technology, Tongji University, Shanghai, 200010, China. 96755@tongji.edu.cn.
Jun ZhangResearch Center for Translational Medicine at East Hospital, School of Medicine, Tongji University, Shanghai, 200010, China. 92870@tongji.edu.cn.
Shanghai East Hospital · CNTongji University · CNTongji Hospital · CN

Funding

the National Natural Science Foundation of China 81771417the Postdoctoral Foundation of China 2022M722409
6 · The paper itself

Abstract

backgroundThe transplantation of exosomes derived from human adipose-derived mesenchymal stem cells (hADSCs) has emerged as a prospective cellular-free therapeutic intervention for the treatment of neurodevelopmental disorders (NDDs), as well as autism spectrum disorder (ASD). Nevertheless, the efficacy of hADSC exosome transplantation for ASD treatment remains to be verified, and the underlying mechanism of action remains unclear.

resultsThe exosomal long non-coding RNAs (lncRNAs) from hADSC and human umbilical cord mesenchymal stem cells (hUCMSC) were sequenced and 13,915 and 729 lncRNAs were obtained, respectively. The lncRNAs present in hADSC-Exos encompass those found in hUCMSC-Exos and are associated with neurogenesis. The biodistribution of hADSC-Exos in mouse brain ventricles and organoids was tracked, and the cellular uptake of hADSC-Exos was evaluated both in vivo and in vitro. hADSC-Exos promote neurogenesis in brain organoid and ameliorate social deficits in ASD mouse model BTBR T + tf/J (BTBR). Fluorescence in situ hybridization (FISH) confirmed lncRNA Ifngas1 significantly increased in the prefrontal cortex (PFC) of adult mice after hADSC-Exos intraventricular injection. The lncRNA Ifngas1 can act as a molecular sponge for miR-21a-3p to play a regulatory role and promote neurogenesis through the miR-21a-3p/PI3K/AKT axis.

conclusionWe demonstrated hADSC-Exos have the ability to confer neuroprotection through functional restoration, attenuation of neuroinflammation, inhibition of neuronal apoptosis, and promotion of neurogenesis both in vitro and in vivo. The hADSC-Exos-derived lncRNA IFNG-AS1 acts as a molecular sponge and facilitates neurogenesis via the miR-21a-3p/PI3K/AKT signaling pathway, thereby exerting a regulatory effect. Our findings suggest a potential therapeutic avenue for individuals with ASD.

Indexed as

Autism Spectrum DisorderExosomesMesenchymal Stem CellsMicroRNAsRNA, Long NoncodingAnimalsHumansIn Situ Hybridization, FluorescenceInterferon-gammaMiceNeurogenesisPhosphatidylinositol 3-KinasesProspective StudiesProto-Oncogene Proteins c-aktTissue DistributionIFNG protein, humanInterferon-gammaMicroRNAsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRNA, Long NoncodinghADSC-ExoshUCMSC-ExoslncRNA IFNG-AS1miR-21a-3pNeurogenesis

Identifiers

PMID38368393
PMCPMC10874555
OpenAlexW4391895811

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.