Evidence map›Paper›PMID 38366549›Full record

ArticleAnnals of botany2024

Analyses of high spatial resolution datasets identify genes associated with multi-layered secondary cell wall thickening in Pinus bungeana.

Yu Guo, Lichao Jiao, Jie Wang, Lingyu Ma, Yang Lu, Yonggang Zhang, Juan Guo, Yafang Yin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of botany, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yu GuoWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Lichao JiaoWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Jie WangWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Lingyu MaWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Yang LuWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Yonggang ZhangWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Juan GuoWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Yafang YinWood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Chinese Academy of Forestry · CN

Funding

Department of Science and Technology, National Forestry and Grassland Administration 2020132601National Natural Science Foundation of China 32071691
6 · The paper itself

Abstract

BACKGROUND AND

aimsSecondary cell wall (SCW) thickening is a major cellular developmental stage determining wood structure and properties. Although the molecular regulation of cell wall deposition during tracheary element differentiation has been well established in primary growth systems, less is known about the gene regulatory processes involved in the multi-layered SCW thickening of mature trees.

methodsUsing third-generation [long-read single-molecule real-time (SMRT)] and second-generation [short-read sequencing by synthesis (SBS)] sequencing methods, we established a Pinus bungeana transcriptome resource with comprehensive functional and structural annotation for the first time. Using these approaches, we generated high spatial resolution datasets for the vascular cambium, xylem expansion regions, early SCW thickening, late SCW thickening and mature xylem tissues of 71-year-old Pinus bungeana trees. KEY

resultsA total of 79 390 non-redundant transcripts, 31 808 long non-coding RNAs and 5147 transcription factors were annotated and quantified in different xylem tissues at all growth and differentiation stages. Furthermore, using this high spatial resolution dataset, we established a comprehensive transcriptomic profile and found that members of the NAC, WRKY, SUS, CESA and LAC gene families are major players in early SCW formation in tracheids, whereas members of the MYB and LBD transcription factor families are highly expressed during late SCW thickening.

conclusionsOur results provide new molecular insights into the regulation of multi-layered SCW thickening in conifers. The high spatial resolution datasets provided can serve as important gene resources for improving softwoods.

Indexed as

Cell WallPinusXylemGene Expression Regulation, PlantGenes, PlantTranscriptomeWoodcambium cellhigh spatial resolutionmature woodPinus bungeanaregulation networkRNA sequencingsecondary cell wall thickeningtranscription factorwood anatomywood formation

Identifiers

PMID38366549
PMCPMC11089263
OpenAlexW4391898686

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.