Evidence map›Paper›PMID 38366179›Full record

ArticleThe ISME journal2024

Commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota.

Ana Popovic, Eric Y Cao, Joanna Han, Nirvana Nursimulu, Eliza V C Alves-Ferreira, Kyle Burrows, Andrea Kennard, Noor Alsmadi, Michael E Grigg, Arthur Mortha and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ISME journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
6.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Evolution and ecology of commensal gut protists: recent advances.Current opinion in genetics & development · 2025
    Review
  4. American journal of physiology. Gastrointestinal and liver physiology · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Tuft cells in the intestine, immunity and beyond.Nature reviews. Gastroenterology & hepatology · 2024
    Review
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Ana PopovicProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, M5G 0A4, Canada.
Eric Y CaoDepartment of Immunology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Joanna HanDepartment of Immunology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Nirvana NursimuluProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, M5G 0A4, Canada.
Eliza V C Alves-FerreiraMolecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
Kyle BurrowsDepartment of Immunology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Andrea KennardMolecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
Noor AlsmadiDepartment of Immunology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Michael E GriggMolecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
Arthur MorthaDepartment of Immunology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
John ParkinsonProgram in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, M5G 0A4, Canada.
University of Toronto · CAHospital for Sick Children · CANational Institutes of Health · USNational Institute of Allergy and Infectious Diseases · US

Funding

Toxoplasma Population GeneticsZIAAI001018 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI GRIGG, MICHAEL · 2009 to 2025
$11.3M
CIHR MRT-168043NIH HHS
6 · The paper itself

Abstract

Commensal protists and gut bacterial communities exhibit complex relationships, mediated at least in part through host immunity. To improve our understanding of this tripartite interplay, we investigated community and functional dynamics between the murine protist Tritrichomonas musculus and intestinal bacteria in healthy and B-cell-deficient mice. We identified dramatic, protist-driven remodeling of resident microbiome growth and activities, in parallel with Tritrichomonas musculus functional changes, which were accelerated in the absence of B cells. Metatranscriptomic data revealed nutrient-based competition between bacteria and the protist. Single-cell transcriptomics identified distinct Tritrichomonas musculus life stages, providing new evidence for trichomonad sexual replication and the formation of pseudocysts. Unique cell states were validated in situ through microscopy and flow cytometry. Our results reveal complex microbial dynamics during the establishment of a commensal protist in the gut, and provide valuable data sets to drive future mechanistic studies.

Indexed as

Gastrointestinal MicrobiomeMicrobiotaTritrichomonasAnimalsBacteriaEukaryotaMiceeukaryotic microbiotamicrobiomepathogenprotistsTritrichomonas

Identifiers

PMID38366179
PMCPMC10944700
OpenAlexW4391672773

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.