Evidence map›Paper›PMID 38365880›Full record

ArticleScientific reports2024

Increased specificity of Fasciola hepatica excretory-secretory antigens combining negative selection on hydroxyapatite and salt precipitation.

Florencio M Ubeira, Marta González-Warleta, Victoria Martínez-Sernández, José Antonio Castro-Hermida, Esperanza Paniagua, Fernanda Romarís, Mercedes Mezo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Florencio M UbeiraLaboratorio de Parasitología, Facultad de Farmacia, 15782, Santiago de Compostela, Spain. fm.ubeira@usc.es.
Marta González-WarletaLaboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, AGACAL, 15318, Abegondo (A Coruña), Spain.
Victoria Martínez-SernándezLaboratorio de Parasitología, Facultad de Farmacia, 15782, Santiago de Compostela, Spain.
José Antonio Castro-HermidaLaboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, AGACAL, 15318, Abegondo (A Coruña), Spain.
Esperanza PaniaguaLaboratorio de Parasitología, Facultad de Farmacia, 15782, Santiago de Compostela, Spain.
Fernanda RomarísLaboratorio de Parasitología, Facultad de Farmacia, 15782, Santiago de Compostela, Spain.
Mercedes MezoLaboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, AGACAL, 15318, Abegondo (A Coruña), Spain.
Universidade de Santiago de Compostela · ES

Funding

Universidad de Santiago de Compostela GI-1219 Research Group funds
6 · The paper itself

Abstract

A single and rapid method to obtain an antigenic fraction of excretory-secretory antigens (ESAs) from Fasciola hepatica suitable for serodiagnosis of fascioliasis is reported. The procedure consists in the negative selection of F. hepatica ESAs by hydroxyapatite (HA) chromatography (HAC; fraction HAC-NR) followed by antigen precipitation with 50% ammonium sulphate (AS) and subsequent recovery by means of a Millex-GV or equivalent filter (Fi-SOLE fraction). Tested in indirect ELISA, the Fi-SOLE antigens detected natural infections by F. hepatica with 100% sensitivity and 98.9% specificity in sheep, and 97.7% sensitivity and 97.7% specificity in cattle, as determined by ROC analysis. The SDS-PAGE and proteomic nano-UHPLC-Tims-QTOF MS/MS analysis of fractions showed that the relative abundance of L-cathepsins and fragments thereof was 57% in fraction HAC-NR and 93.8% in fraction Fi-SOLE. The second most abundant proteins in fraction HAC-NR were fatty-acid binding proteins (11.9%). In contrast, free heme, and heme:MF6p/FhHDM-1 complexes remained strongly bond to the HA particles during HAC. Interestingly, phosphorylcholine (PC)-bearing antigens, which are a frequent source of cross-reactivity, were detected with an anti-PC mAb (BH8) in ESAs and fraction HAC-NR but were almost absent in fraction Fi-SOLE.

Indexed as

Fasciola hepaticaFascioliasisSheep DiseasesAnimalsAntibodies, HelminthAntigens, HelminthCattleEnzyme-Linked Immunosorbent AssayHemeHydroxyapatitesProteomicsSensitivity and SpecificitySheepTandem Mass SpectrometryAntibodies, HelminthAntigens, HelminthHemeHydroxyapatites

Identifiers

PMID38365880
PMCPMC10873304
OpenAlexW4391883945

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.