ArticleNature communications2024
Identification of structural and regulatory cell-shape determinants in Haloferax volcanii.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- The halophilic archaeonMicrobiology spectrum · 2026Article
- Characterization of aliA and aliB deletion mutants reveals a dominant role of AliA in Haloferax volcanii lipoprotein lipidation.The Journal of biological chemistry · 2026Article
- Article
- CsmR controls both, motility and cell shape, in Haloferax volcanii.PLoS genetics · 2026Article
- Cytoskeletal prestress homeostasis is a biological principle that governs living cell structure and function.Mechanobiology in medicine · 2026Review
- Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins inmicroLife · 2026Article
- A Two-Component Regulatory System Mediates Quorum Sensing-Dependent Morphology and Motility Transitions in the ArchaeonbioRxiv : the preprint server for biology · 2025Article
- Uncovering the prevalence, key biogenesis enzymes, and biological significance of archaeal lipoproteins.Nature communications · 2025Article
- Article
- Rapid rewiring of an archaeal transcription factor function via flexible cis-trans interactions.Molecular biology of the cell · 2025Article
- Review
- Decoding the Chemical Language of Ribosomally Synthesized and Post-Translationally Modified Peptides from the Untapped Archaea Domain.Angewandte Chemie (International ed. in English) · 2025Article
- Cell division protein CdpA organises and anchors the midcell ring in haloarchaea.Nature communications · 2025Article
- Tissue-like multicellular development triggered by mechanical compression in archaea.Science (New York, N.Y.) · 2025Article
- Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.mSystems · 2025Article
- Provirus deletion frommicroLife · 2025Article
- Halofilins as emerging bactofilin families of archaeal cell shape plasticity orchestrators.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- MinD2 modulates cell shape and motility in the archaeonbioRxiv : the preprint server for biology · 2024Article
- Intermolecular Gene Conversion for the Equalization of Genome Copies in the Polyploid HaloarchaeonGenes · 2024Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Archaea play indispensable roles in global biogeochemical cycles, yet many crucial cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used a combination of iterative proteomics, genetics, and live-cell imaging to identify mutants that only form rods or disks. We compared the proteomes of the mutants with wild-type cells across growth phases, thereby distinguishing between protein abundance changes specific to cell shape and those related to growth phases. The results identified a diverse set of proteins, including predicted transporters, transducers, signaling components, and transcriptional regulators, as important for cell-shape determination. Through phenotypic characterization of deletion strains, we established that rod-determining factor A (RdfA) and disk-determining factor A (DdfA) are required for the formation of rods and disks, respectively. We also identified structural proteins, including an actin homolog that plays a role in disk-shape morphogenesis, which we named volactin. Using live-cell imaging, we determined volactin's cellular localization and showed its dynamic polymerization and depolymerization. Our results provide insights into archaeal cell-shape determination, with possible implications for understanding the evolution of cell morphology regulation across domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.