Evidence map›Paper›PMID 38355914›Full record

ArticleJournal of neurovirology2024

HIV-1 mRNA knockdown with CRISPR/CAS9 enhances neurocognitive function.

Kristen A McLaurin, Hailong Li, Kamel Khalili, Charles F Mactutus, Rosemarie M Booze

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurovirology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Kristen A McLaurinCognitive and Neural Science Program, Department of Psychology, Barnwell College, University of South Carolina, 1512 Pendleton Street, Columbia, SC, 29208, USA.
Hailong LiCognitive and Neural Science Program, Department of Psychology, Barnwell College, University of South Carolina, 1512 Pendleton Street, Columbia, SC, 29208, USA.
Kamel KhaliliCenter for Neurovirology and Gene Editing, Department of Microbiology, Immunology, and Inflammation, Lewis Katz School of Medicine, Temple University, 3500 N. Broad Street, 7th Floor, Philadelphia, PA, 19140, USA.
Charles F MactutusCognitive and Neural Science Program, Department of Psychology, Barnwell College, University of South Carolina, 1512 Pendleton Street, Columbia, SC, 29208, USA.
Rosemarie M BoozeCognitive and Neural Science Program, Department of Psychology, Barnwell College, University of South Carolina, 1512 Pendleton Street, Columbia, SC, 29208, USA. booze@mailbox.sc.edu.
University of South Carolina · USTemple University · USUniversity of Kentucky · US

Funding

HIV/COCAINE NEUROTOXICITY IN FEMALESR01DA013137 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI BOOZE, ROSEMARIE M, MACTUTUS, CHARLES F. · 2000 to 2021
$7.7M
Maternal HIV: Developmental Neurotoxicity - Administrative SupplementR01HD043680 · NICHD · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI BOOZE, ROSEMARIE M, MACTUTUS, CHARLES F. · 2003 to 2016
$2.8M
Microglial modulation of neurocircuits in HIV/cocaine comorbidityR01DA059310 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI Rosemarie M Booze · 2023 to 2026
$2.0M
Progression of Attentional and Executive Function Deficits in HANDR01MH106392 · NIMH · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI BOOZE, ROSEMARIE M, MACTUTUS, CHARLES F. · 2016 to 2020
$1.8M
Neurobiological Mechanisms of Apathy in HANDR01NS100624 · NINDS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI BOOZE, ROSEMARIE M, MACTUTUS, CHARLES F. · 2017 to 2021
$1.8M
Neurocognitive Impairments Resulting from Adolescent Prescription Opioid Use Disorder: Longitudinal Impact, Neural Mechanisms, and ComorbiditiesR00DA056288 · NIDA · UNIVERSITY OF KENTUCKY · PI Kristen Addie McLaurin · 2024 to 2026
$747k
Neurocognitive Impairments Resulting from Adolescent Prescription Opioid Use Disorder: Longitudinal Impact, Neural Mechanisms, and ComorbiditiesK99DA056288 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI MCLAURIN, KRISTEN ADDIE · 2022 to 2023
$262k
NICHD NIH HHS R01 HD043680NIDA NIH HHS DA056288NIDA NIH HHS DA059310NIDA NIH HHS K99 DA056288NIDA NIH HHS R00 DA056288NIDA NIH HHS R01 DA013137NIDA NIH HHS R01 DA059310NIMH NIH HHS MH106392NIMH NIH HHS R01 MH106392NINDS NIH HHS NS100624NINDS NIH HHS R01 NS100624
6 · The paper itself

Abstract

Mixed glia are infiltrated with HIV-1 virus early in the course of infection leading to the development of a persistent viral reservoir in the central nervous system. Modification of the HIV-1 genome using gene editing techniques, including CRISPR/Cas9, has shown great promise towards eliminating HIV-1 viral reservoirs; whether these techniques are capable of removing HIV-1 viral proteins from mixed glia, however, has not been systematically evaluated. Herein, the efficacy of adeno-associated virus 9 (AAV9)-CRISPR/Cas9 gene editing for eliminating HIV-1 messenger RNA (mRNA) from cortical mixed glia was evaluated in vitro and in vivo. In vitro, a within-subjects experimental design was utilized to treat mixed glia isolated from neonatal HIV-1 transgenic (Tg) rats with varying doses (0, 0.9, 1.8, 2.7, 3.6, 4.5, or 5.4 µL corresponding to a physical titer of 0, 4.23 × 10

Indexed as

CRISPR-Cas SystemsGene EditingHIV-1Rats, TransgenicRNA, MessengerAnimalsCognitionDependovirusGene Knockdown TechniquesHIV InfectionsHumansNeurogliaRatsRNA, ViralRNA, MessengerRNA, ViralCell cultureGene editingIn situ hybridizationMicrogliaPrepulse inhibition

Identifiers

PMID38355914
PMCPMC11035469
OpenAlexW4391806768

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.