Evidence map›Paper›PMID 38352439›Full record

ArticlebioRxiv : the preprint server for biology2024

Understanding species-specific and conserved RNA-protein interactions

Sarah E Harris, Maria S Alexis, Gilbert Giri, Francisco F Cavazos, Jernej Murn, Maria M Aleman, Christopher B Burge, Daniel Dominguez

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Sarah E HarrisDepartment of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC.ORCID 0000-0002-4807-2578
Maria S AlexisDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA.
Gilbert GiriDepartment of Pharmacology, University of North Carolina, Chapel Hill, NC.
Francisco F CavazosDepartment of Pharmacology, University of North Carolina, Chapel Hill, NC.
Jernej MurnDepartment of Biochemistry, University of California, Riverside, CA.
Maria M AlemanDepartment of Pharmacology, University of North Carolina, Chapel Hill, NC.
Christopher B BurgeDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA.
Daniel DominguezDepartment of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC.
University of North Carolina at Chapel Hill · USEpix Pharmaceuticals (United States) · USMassachusetts Institute of Technology · USUniversity of California, Riverside · US

Funding

MOLECULAR AND CELLULAR BIOPHYSICS TRAINING PROGRAMT32GM008570 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI KUHLMAN, BRIAN A, SLEP, KEVIN C · 1995 to 2022
$5.1M
Protein Disorder as a Modifier of RNA Binding and RegulationR35GM142864 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Daniel Issac Dominguez · 2021 to 2026
$2.5M
NIGMS NIH HHS R35 GM142864NIGMS NIH HHS T32 GM008570
6 · The paper itself

Abstract

While evolution is often considered from a DNA- and protein-centric view, RNA-based regulation can also impact gene expression and protein sequences. Here we examined interspecies differences in RNA-protein interactions using the conserved neuronal RNA binding protein, Unkempt (UNK) as model. We find that roughly half of mRNAs bound in human are also bound in mouse. Unexpectedly, even when transcript-level binding was conserved across species differential motif usage was prevalent. To understand the biochemical basis of UNK-RNA interactions, we reconstituted the human and mouse UNK-RNA interactomes using a high-throughput biochemical assay. We uncover detailed features driving binding, show that

Identifiers

PMID38352439
PMCPMC10862761
OpenAlexW4391349439

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.