Evidence map›Paper›PMID 38352317›Full record

ArticlebioRxiv : the preprint server for biology2024

Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects of

Sen Xu, Swatantra Neupane, Hongjun Wang, Thinh Phu Pham, Marelize Snyman, Trung V Huynh, Li Wang

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sen XuDivision of Biological Sciences, University of Missouri, Columbia, Missouri, 65211, USA.ORCID 0000-0002-1304-9968
Swatantra NeupaneDivision of Biological Sciences, University of Missouri, Columbia, Missouri, 65211, USA.
Hongjun WangDepartment of Biology, University of Texas at Arlington, Arlington, Texas, 76019, USA.
Thinh Phu PhamDepartment of Biology, University of Texas at Arlington, Arlington, Texas, 76019, USA.
Marelize SnymanDepartment of Biology, University of Texas at Arlington, Arlington, Texas, 76019, USA.ORCID 0000-0002-1051-3008
Trung V HuynhDivision of Biological Sciences, University of Missouri, Columbia, Missouri, 65211, USA.
Li WangDivision of Biological Sciences, University of Missouri, Columbia, Missouri, 65211, USA.
University of Missouri · USThe University of Texas at Arlington · US

Funding

Understanding the origin of parthenogenesisR35GM133730 · NIGMS · UNIVERSITY OF TEXAS ARLINGTON · PI Sen Xu · 2019 to 2026
$2.7M
NIGMS NIH HHS R35 GM133730
6 · The paper itself

Abstract

Despite the revolutionary impacts of CRISPR-Cas gene editing systems, the effective and widespread use of CRISPR technologies in emerging model organisms still faces significant challenges. These include the inefficiency in generating heritable mutations at the organismal level, limited knowledge about the genomic consequences of gene editing, and an inadequate understanding of the inheritance patterns of CRISPR-Cas-induced mutations. This study addresses these issues by 1) developing an efficient microinjection delivery method for CRISPR editing in the microcrustacean

Indexed as

Casknock-inmutationoff-target mutationswimming behavior

Identifiers

PMID38352317
PMCPMC10862705
OpenAlexW4391378676

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.